Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Elongation factor 1-gamma antibody [EPR7200] - BSA and Azide free (ab248088)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7200] to Elongation factor 1-gamma - BSA and Azide free
  • Suitable for: ICC, Flow Cyt, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Elongation factor 1-gamma antibody [EPR7200] - BSA and Azide free
    See all Elongation factor 1-gamma primary antibodies

  • Description

    Rabbit monoclonal [EPR7200] to Elongation factor 1-gamma - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, Flow Cyt, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248088 is the carrier-free version of ab124994. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248088 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Elongation factor 1-gamma antibody [EPR7200] - BSA and Azide free (ab248088)
    Western blot - Anti-Elongation factor 1-gamma antibody [EPR7200] - BSA and Azide free (ab248088)
    All lanes : Anti-Elongation factor 1-gamma antibody [EPR7200] (ab124994) at 1/10000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HL-60 cell lysate
    Lane 3 : C6 cell lysate
    Lane 4 : RAW 264.7 cell lysate
    Lane 5 : PC-12 cell lysate
    Lane 6 : NIH/3T3 cell lysate
    Lane 7 : HepG2 cell lysate
    Lane 8 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP-conjugated at 1/2000 dilution

    Predicted band size: 50 kDa



    This data was developed using ab124994, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-Elongation factor 1-gamma antibody [EPR7200] - BSA and Azide free (ab248088)
    Flow Cytometry - Anti-Elongation factor 1-gamma antibody [EPR7200] - BSA and Azide free (ab248088)
    This data was developed using ab124994, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with ab124994 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124994, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elongation factor 1-gamma antibody [EPR7200] - BSA and Azide free (ab248088)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elongation factor 1-gamma antibody [EPR7200] - BSA and Azide free (ab248088)
    This data was developed using ab124994, the same antibody clone in a different buffer formulation.ab124994 at 1/250 dilution staining Elongation factor 1-gamma in paraffin-embedded Human pancreatic adenocarcinoma tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-Elongation factor 1-gamma antibody [EPR7200] - BSA and Azide free (ab248088)
    Immunocytochemistry - Anti-Elongation factor 1-gamma antibody [EPR7200] - BSA and Azide free (ab248088)
    This data was developed using ab124994, the same antibody clone in a different buffer formulation.ab124994 at 1/100 dilution staining Elongation factor 1-gamma in HeLa cells by Immunofluorescence.
  • OI-RD Scanning - Anti-Elongation factor 1-gamma antibody [EPR7200] - BSA and Azide free (ab248088)
    OI-RD Scanning - Anti-Elongation factor 1-gamma antibody [EPR7200] - BSA and Azide free (ab248088)
    This data was developed using ab124994, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Elongation factor 1-gamma antibody [EPR7200] - BSA and Azide free (ab248088)
    Anti-Elongation factor 1-gamma antibody [EPR7200] - BSA and Azide free (ab248088)

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