Anti-ELK1 antibody [EPR1913(2)] - BSA and Azide free (ab250948)
![Anti-ELK1 antibody [EPR1913(2)] - BSA and Azide free (ab250948)](https://www.abcam.com/ps/products/250/ab250948/Images/ab250948-447944-anti-elk1-antibody-epr19132-western-blot-hela-lysates.jpg "Anti-ELK1 antibody [EPR1913(2)] - BSA and Azide free (ab250948)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1913(2)] to ELK1 - BSA and Azide free
- Suitable for: ICC, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-ELK1 antibody [EPR1913(2)] - BSA and Azide free
See all ELK1 primary antibodies -
Description
Rabbit monoclonal [EPR1913(2)] to ELK1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and K562 cell lysate. ICC: HeLa cells.
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General notes
Ab250948 is the carrier-free version of ab188316. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250948 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR1913(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ELK1 antibody [EPR1913(2)] - BSA and Azide free (ab250948)All lanes : Anti-ELK1 antibody [EPR1913(2)] (ab188316) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ELK1 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 45 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?This data was developed using the same antibody clone in a different buffer formulation (ab188316).
Lanes 1- 2: Merged signal (red and green). Green - ab188316 observed at 55 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab188316 was shown to react with ELK1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261764 (knockout cell lysate ab256904) was used. Wild-type HeLa and ELK1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab188316 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry - Anti-ELK1 antibody [EPR1913(2)] - BSA and Azide free (ab250948)](https://www.abcam.com/ps/products/250/ab250948/Images/ab250948-2-anti-elk1-antibody-epr1913-2-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-ELK1 antibody [EPR1913(2)] - BSA and Azide free (ab250948)This data was developed using ab188316, the same antibody clone in a different buffer formulation.Immunocytochemical analysis of HeLa cells fixed in 2% paraformaldehyde labeling ELK1 with ab188316 at 1/100 dilution and Goat anti rabbit IgG(Alexa Fluor® 488) at 1/200 dilution. Counterstained with DAPI (blue). -
![Western blot - Anti-ELK1 antibody [EPR1913(2)] - BSA and Azide free (ab250948)](https://www.abcam.com/ps/products/250/ab250948/Images/ab250948-406442-anti-elk1-antibody-epr1913-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ELK1 antibody [EPR1913(2)] - BSA and Azide free (ab250948)This data was developed using ab188316, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: ELK1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa positve whole cell lysate (20 µg)
Lane 4: MCF7 negative whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab188316 observed at 55 kDa. Red - loading control, ab18058, observed at 130 kDa.
ab188316 was shown to specifically react with ELK1 in wild-type HAP1 cells as signal was lost in ELK1 knockout cells. Wild-type and ELK1 knockout samples were subjected to SDS-PAGE. ab188316 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ELK1 antibody [EPR1913(2)] - BSA and Azide free (ab250948)](https://www.abcam.com/ps/products/250/ab250948/Images/ab250948-398982-anti-elk1-antibody-epr1913-2-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ELK1 antibody [EPR1913(2)] - BSA and Azide free (ab250948)All lanes : Anti-ELK1 antibody [EPR1913(2)] (ab188316) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDaThis data was developed using ab188316, the same antibody clone in a different buffer formulation.
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![Anti-ELK1 antibody [EPR1913(2)] - BSA and Azide free (ab250948)](https://www.abcam.com/ps/products/250/ab250948/Images/ab250948-4-benefits-of-recombinant-antibodies.png)
Anti-ELK1 antibody [EPR1913(2)] - BSA and Azide free (ab250948)