Anti-Elastin antibody (ab217356)
Key features and details
- Rabbit polyclonal to Elastin
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Elastin antibody
See all Elastin primary antibodies -
Description
Rabbit polyclonal to Elastin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Elastin aa 750 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P15502-3 -
Positive control
- IHC-P: Human artery and glioma tissues; rat intervertebral disk tissue; mouse heart tissues. WB: Mouse lung and intestine lysates, rat lung lysates. FCM: A549 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody (ab217356)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human artery tissue labeling Elastin with ab217356 at 1/100 dilution followed by conjugation to the secondary antibody and DAB staining.
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Western blot - Anti-Elastin antibody (ab217356)All lanes : Anti-Elastin antibody (ab217356) at 1/200 dilution
Lane 1 : mouse intestine lysate
Lane 2 : rat lung lysate
Secondary
All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution
Predicted band size: 68 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody (ab217356)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human glioma tissue labeling Elastin with ab217356 at 1/100 dilution followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody (ab217356)Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat intervertebral disk tissue labeling Elastin with ab217356 at 1/100 dilution followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Elastin antibody (ab217356)Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse heart tissue labeling Elastin with ab217356 at 1/1000 dilution followed by conjugation to the secondary antibody.
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Flow Cytometry - Anti-Elastin antibody (ab217356)Flow cytometric analysis of A549 cells labeling Elastin with ab217356 at 1/50 dilution for 40 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary at 1/100 (green) for 40 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
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Western blot - Anti-Elastin antibody (ab217356)Anti-Elastin antibody (ab217356) at 1/300 dilution + mouse lung lysate
Secondary
conjugated secondary at 1/10000 dilution
Predicted band size: 68 kDa
