Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free (ab251187)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18667] to EHMT2/G9A+EHMT1/GLP - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free
    See all EHMT2/G9A+EHMT1/GLP primary antibodies

  • Description

    Rabbit monoclonal [EPR18667] to EHMT2/G9A+EHMT1/GLP - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, ICC, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251187 is the carrier-free version of ab194299. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251187 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free (ab251187)
    Western blot - Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free (ab251187)
    Anti-EHMT2/G9A + EHMT1/GLP antibody [EPR18667] (ab194299) at 1/1000 dilution + Human fetal kidney lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 141, 132 kDa
    Observed band size: 170 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    This data was developed using ab194299, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free (ab251187)
    Flow Cytometry - Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free (ab251187)

    This data was developed using ab194299, the same antibody clone in a different buffer formulation.

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling EHMT2/G9A + EHMT1/GLP (red) with purified ab194299 at a dilution of 1/150. Goat anti rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/2000. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Isotype control antibody was Rabbit monoclonal IgG (black). The blue line shows cells without incubation with primary antibody and secondary antibody.
  • Western blot - Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free (ab251187)
    Western blot - Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free (ab251187)
    All lanes : Anti-EHMT2/G9A + EHMT1/GLP antibody [EPR18667] (ab194299) at 1/5000 dilution

    Lane 1 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 141, 132 kDa
    Observed band size: 170 kDa why is the actual band size different from the predicted?



    This data was developed using ab194299, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 20 seconds; Lane 3: 30 seconds.

  • Western blot - Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free (ab251187)
    Western blot - Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free (ab251187)
    Lane 1 : Anti-EHMT2/G9A + EHMT1/GLP antibody [EPR18667] (ab194299) at 1/5000 dilution


    All lanes : EHMT2 recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 141, 132 kDa
    Observed band size: 30,60 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    This data was developed using ab194299, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    EHMT2 recombinant protein fragment with His-Tag®.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free (ab251187)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free (ab251187)
    This data was developed using ab194299, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling EHMT2/G9A + EHMT1/GLP with ab194299 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on human tonsil is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free (ab251187)
    Immunocytochemistry - Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free (ab251187)
    This data was developed using ab194299, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling EHMT2/G9A + EHMT1/GLP with ab194299 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue).Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).The negative controls are as follows:
    -ve control 1: ab194299 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
  • Immunocytochemistry - Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free (ab251187)
    Immunocytochemistry - Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free (ab251187)
    This data was developed using ab194299, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling EHMT1/GLP + EHMT2/G9A with ab194299 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on NIH/3T3. The nuclear counter stain is DAPI (blue).Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab194299 at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary at 1/1000 dilution.
  • Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free (ab251187)
    Anti-EHMT2/G9A+EHMT1/GLP antibody [EPR18667] - BSA and Azide free (ab251187)

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