Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EGFR (phospho Y1173) antibody [E124] - BSA and Azide free (ab203559)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E124] to EGFR (phospho Y1173) - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-EGFR (phospho Y1173) antibody [E124] - BSA and Azide free
    See all EGFR primary antibodies

  • Description

    Rabbit monoclonal [E124] to EGFR (phospho Y1173) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: EGF-treated A431 cells lysate IHC: Phosphatase-treated, paraffin-embedded skin carcinoma

  • General notes

    ab203559 is the carrier-free version of ab32578 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab203559 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-EGFR (phospho Y1173) antibody [E124] - BSA and Azide free (ab203559)
    Flow Cytometry - Anti-EGFR (phospho Y1173) antibody [E124] - BSA and Azide free (ab203559)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) treated (Red)/untreated (Green) with 500ng/ml Trichostatin A for 4 hours with purified ab177178 at 1/1300 dilution. The secondary antibody was Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution. A Rabbit monoclonal IgG (Black) was used as the isotype control and cells without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32578).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y1173) antibody [E124] - BSA and Azide free (ab203559)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y1173) antibody [E124] - BSA and Azide free (ab203559)

    ab32578 (at 1/50) staining (A) untreated and (B) Phosphatase-treated skin carcinoma tissue by IHC-P.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32578).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1173) antibody [E124] - BSA and Azide free (ab203559)
    Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1173) antibody [E124] - BSA and Azide free (ab203559)
    Immunofluorescent staining of untreated A431 cells using ab32578.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32578).

  • Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1173) antibody [E124] - BSA and Azide free (ab203559)
    Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1173) antibody [E124] - BSA and Azide free (ab203559)
    Immunofluorescent staining of Phosphatase-treated A431 cells using ab32578.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32578).

  • Anti-EGFR (phospho Y1173) antibody [E124] - BSA and Azide free (ab203559)
    Anti-EGFR (phospho Y1173) antibody [E124] - BSA and Azide free (ab203559)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-EGFR (phospho Y1173) antibody [E124] - BSA and Azide free (ab203559)

© 2021 Astana Biomed Group LLP. All rights reserved.