Anti-EGFR (phospho Y1173) antibody (ab34901)
Key features and details
- Rabbit polyclonal to EGFR (phospho Y1173)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EGFR (phospho Y1173) antibody
See all EGFR primary antibodies -
Description
Rabbit polyclonal to EGFR (phospho Y1173) -
Host species
Rabbit -
Specificity
Less than 5.0% reactivity is observed against the non-phosphorylated form of the immunizing peptide. Recognises EGFR phosphorylated on Tyrosine 1173 of the mature human isoform 1 (corresponding to Y1173 from the precursor form P00533-1/p170) -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human EGFR aa 1189-1199.
Sequence:GSTAENAEYLR
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The product was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-EGFR (phospho Y1173) antibody (ab34901)All lanes : Anti-EGFR (phospho Y1173) antibody (ab34901) at 2.2 µg/ml
Lane 1 : A431 whole cell lysate
Lane 2 : A431 whole cell lysate, stimulated with EGF (50 ng/ml for 15 min)
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Near infrared fluorescent dye conjugated Goat anti-Rabbit IgG
Predicted band size: 170 kDa
Observed band size: 170 kDaLane M: Prestained MW markers (700nm channel, red)
Reaction with primary antibody occured overnight at 4°C followed by washes and reaction with secondary antibody for 45 min at room temperature (800nm channel, green)
Fluorescent image was captured using an infrared image detection system.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y1173) antibody (ab34901)ab34901 at 5 µg/ml staining placental trophoblasts at 40X. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
