Antibodies, Proteins, Kits and Reagents for Life Science

Anti-EGFR (phospho Y1068) antibody [SP353] - BSA and Azide free (ab242417)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP353] to EGFR (phospho Y1068) - BSA and Azide free
Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
Reacts with: Human

Product name

Anti-EGFR (phospho Y1068) antibody [SP353] - BSA and Azide free
See all EGFR primary antibodies
Description

Rabbit monoclonal [SP353] to EGFR (phospho Y1068) - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide within Human EGFR (phospho Y1068). The exact sequence is proprietary.
Database link: P00533
Positive control
- IHC-P: Human lung cancer tissue. Flow Cyt.: A431 and EGF cells. WB: A431 and EGF cell lysate. ICC/IF: A431 cells
General notes
ab242417 is the carrier-free version of ab223499.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.20
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

SP353
Isotype

IgG
Research areas
- Neuroscience
- Development
- Neuroscience
- Processes

Immunocytochemistry/ Immunofluorescence - Anti-EGFR (phospho Y1068) antibody [SP353] - BSA and Azide free (ab242417)

Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling EGFR with purified ab223499 at 1:100 (4.3 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242417)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y1068) antibody [SP353] - BSA and Azide free (ab242417)

PFA-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for EGFR using ab223499 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223499)
Flow Cytometry - Anti-EGFR (phospho Y1068) antibody [SP353] - BSA and Azide free (ab242417)

Flow Cytometry analysis of A431 cells treated with 100ng/ml EGF for 10mins cells labeling EGFR with purified ab223499 at 1:4200 dilution (0.10 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. Untreated cells - GreenThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab242417)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EGFR (phospho Y1068) antibody [SP353] - BSA and Azide free (ab242417)

PFA-fixed, paraffin-embedded human lung cancer tissue stained for EGFR using ab223499 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223499)
Flow Cytometry - Anti-EGFR (phospho Y1068) antibody [SP353] - BSA and Azide free (ab242417)

Flow cytometric analysis of ab223499 in A431 (human epidermoid carcinoma cell line) + EGF cells (green) at 1/400 dilution compared to negative control of rabbit IgG (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab223499)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com