Anti-Insulin Receptor beta antibody [C18C4] (ab69508)
![Anti-Insulin Receptor beta antibody [C18C4] (ab69508)](https://www.abcam.com/ps/products/69/ab69508/Images/ab69508-274574-2.jpg "Anti-Insulin Receptor beta antibody [C18C4] (ab69508)")
Key features and details
- Mouse monoclonal [C18C4] to Insulin Receptor beta
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Insulin Receptor beta antibody [C18C4]
See all Insulin Receptor beta primary antibodies -
Description
Mouse monoclonal [C18C4] to Insulin Receptor beta -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB MouseRatHuman
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Immunogen
Recombinant fragment corresponding to Human Insulin Receptor beta.
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Positive control
- MCF-7, Rat-2 and L-929 cells
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General notes
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
C18C4 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Insulin Receptor beta antibody [C18C4] (ab69508)All lanes : Anti-Insulin Receptor beta antibody [C18C4] (ab69508) at 1/1000 dilution
Lane 2 : HeLa lysate (Human epithelial cell line from cervix adenocarcinoma)
Lane 3 : MCF-7 lysate (Human breast adenocarcinoma cell line)
Lane 4 : PC-12 lysate (Rat adrenal gland pheochromocytoma cell line)
Lane 5 : 3T3-L1 lysate (Mouse embryonic fibroblast cell line)
Developed using the ECL technique.
Predicted band size: 95 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?This image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor beta antibody [C18C4] (ab69508)](https://www.abcam.com/ps/products/69/ab69508/Images/Insulin-Receptor-beta-Primary-antibodies-ab69508-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Insulin Receptor beta antibody [C18C4] (ab69508)IHC image of ab69508 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab69508, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-Insulin Receptor beta antibody [C18C4] (ab69508)](https://www.abcam.com/ps/products/69/ab69508/Images/Insulin-Receptor-beta-Primary-antibodies-ab69508-2.jpg)
Flow Cytometry - Anti-Insulin Receptor beta antibody [C18C4] (ab69508)Overlay histogram showing HepG2 cells stained with ab69508 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69508, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.This image was generated using the ascites version of the product.
