Anti-EGFR (phospho Y1045) antibody [11C2] (ab24928)
![Anti-EGFR (phospho Y1045) antibody [11C2] (ab24928)](https://www.abcam.com/ps/products/24/ab24928/Images/ab24928_1.jpg "Anti-EGFR (phospho Y1045) antibody [11C2] (ab24928)")
Key features and details
- Mouse monoclonal [11C2] to EGFR (phospho Y1045)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-EGFR (phospho Y1045) antibody [11C2]
See all EGFR primary antibodies -
Description
Mouse monoclonal [11C2] to EGFR (phospho Y1045) -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human EGFR (phospho Y1045) conjugated to keyhole limpet haemocyanin.
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General notes
The phosphorylation status of Tyrosine 1045 regulates the degradation of activated EGFR. Autophosphorylation of Tyrosine 1045 creates a docking site for the c-cbl ubiquitination ligase.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 2.68% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purification notes
Thiophilic adsorption and size exclusion chromatography. -
Primary antibody notes
The phosphorylation status of Tyrosine 1045 regulates the degradation of activated EGFR. Autophosphorylation of Tyrosine 1045 creates a docking site for the c-cbl ubiquitination ligase. -
Clonality
Monoclonal -
Clone number
11C2 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-EGFR (phospho Y1045) antibody [11C2] (ab24928)All lanes : Anti-EGFR (phospho Y1045) antibody [11C2] (ab24928) at 0.5 µg/ml (EGFR Activation:
Serum starved MDA-MB 468 cells were incubated with 10ng/ml EGF for the indicated times. Whole cell lysates were prepared and separated by SDS-PAGE)
Lane 1 : control
Lane 2 : 5 min EGF
Lane 3 : 15 min EGF
Lane 4 : 30 min EGF
Lane 5 : 1 hour EGF
Lane 6 : 2 hours EGF
Lane 7 : 4 hours EGF
Lane 8 : 8 hours EGF
Lysates/proteins at 20000 cells per lane.
Secondary
All lanes : A HRP-conjugated antibody
Developed using the ECL technique.
Predicted band size: 134 kDa
Exposure time: 30 seconds
Recommended blocking buffer CPPT: 0.5% (w/v) casein, 1% (w/v) PEG 4000, 1% (w/v) Polyvinylpyrrolidone (PVP), 0.1% Tween 20, 10mM Tris/HCl, pH 7.4, 150mM Sodium chloride.
