Anti-EGFP antibody [F56-6A1.2.3] (ab184601)
![Anti-EGFP antibody [F56-6A1.2.3] (ab184601)](https://www.abcam.com/ps/products/184/ab184601/Images/ab184601-336809-anti-egfp-antibody-f56-6a123-immunofluorescence.jpg "Anti-EGFP antibody [F56-6A1.2.3] (ab184601)")
Key features and details
- Mouse monoclonal [F56-6A1.2.3] to EGFP
- Suitable for: WB, ICC/IF
- Reacts with: Species independent
- Isotype: IgG2b
Overview
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Product name
Anti-EGFP antibody [F56-6A1.2.3] -
Description
Mouse monoclonal [F56-6A1.2.3] to EGFP -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Species independentWB Species independent
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Immunogen
Full length protein corresponding to EGFP aa 1-239. enhanced green fluorescent protein (eGFP).
Database link: CAD97424.1 -
Positive control
- ICC/IF: U-2 OS cells transfected with an EGFR-eGFP fusion protein. WB: Recombinant GFP.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
in vitro produced -
Clonality
Monoclonal -
Clone number
F56-6A1.2.3 -
Isotype
IgG2b -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-EGFP antibody [F56-6A1.2.3] (ab184601) Zhang et al PLoS One. 2014 Sep 15;9(9):e105986. doi: 10.1371/journal.pone.0105986. eCollection 2014. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Detection of GP64 expression and fusogenicity.
(B) Syncytium formation assay of BmN-SWU1 or BmN-SWU2 cells transfected with 1 µg pIZ-GP64 plasmid. At 48 h p.t., cells were incubated in TC-100 medium at a normal (6.19) or low (4.8) pH level for 10 min. Syncytium formation was examined 12 h later by detecting GP64 and EGFP (ab184601) using indirect immunofluorescence microscopy. Nuclei were stained with DAPI (blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-EGFP antibody [F56-6A1.2.3] (ab184601)](https://www.abcam.com/ps/products/184/ab184601/Images/ab184601-215230-anti-egfp-antibody-f56-6a123-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-EGFP antibody [F56-6A1.2.3] (ab184601)Immunofluorescent analysis of U-2 OS (Human bone osteosarcoma epithelial cell line) cells transfected with an EGFR-EGFP fusion protein (formalin-fixed, 0.1% Triton X-100 permeabilized) using either natural fluorescence (green) or an anti-EGFP antibody (red). Cells were then labeled with ab184601 at 1/20 dilution followed with DyLight 550 goat anti-mouse IgG secondary antibody at 1/200 dilution. Nuclei (blue) were stained with Hoechst 33342 dye. 20X magnification.
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![Western blot - Anti-EGFP antibody [F56-6A1.2.3] (ab184601)](https://www.abcam.com/ps/products/184/ab184601/Images/ab184601-215231-anti-egfp-antibody-f56-6a123-western-blot.jpg)
Western blot - Anti-EGFP antibody [F56-6A1.2.3] (ab184601)All lanes : Anti-EGFP antibody [F56-6A1.2.3] (ab184601) at 1/1000 dilution
Lane 1 : Recombinant GFP at 1 µg
Lane 2 : Recombinant GFP at 0.5 µg
Lane 3 : Recombinant GFP at 0.25 µg
Lane 4 : Recombinant GFP at 0.125 µg
Lane 5 : Recombinant GFP at 0.0625 µg
Secondary
All lanes : goat anti-mouse IgG-HRP at 1/15000 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa