Anti-EFHC1 antibody (ab80861)
Key features and details
- Rabbit polyclonal to EFHC1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-EFHC1 antibody
See all EFHC1 primary antibodies -
Description
Rabbit polyclonal to EFHC1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Mouse EFHC1 aa 550 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab93070) -
Positive control
- This antibody gave a positive signal in the following mouse tissue lysates: Hippocampus; Cortex.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80861 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseApplication Abreviews Notes WB Notes Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 77 kDa (predicted molecular weight: 75 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
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Function
Necessary for radial and tangential cell migration during brain development, possibly acting as a regulator of cell morphology and process formation during migration (PubMed:22926142). May enhance calcium influx through CACNA1E and stimulate programmed cell death (PubMed:15258581). -
Tissue specificity
Widely expressed. Not detected in lymphocytes. -
Involvement in disease
Juvenile myoclonic epilepsy 1
Juvenile absence epilepsy 1
Mutation Leu-229 may be a cause of intractable epilepsy of infancy. Affected individuals have seizures of multiple type, manifested as tonic, clonic, and myoclonic seizures in the neonatal period, and as tonic seizures activated frequently by sleep, and repeated frequent myoclonic seizures in later infancy. The seizures are unresponsive to numerous antiepileptic drugs, and infants die in the first years of life. Although heterozygosity for Leu-229 has been associated with relatively benign forms of epilepsy in adolescence, homozygosity for the same mutation has much more severe consequences. -
Sequence similarities
Contains 3 DM10 domains.
Contains 1 EF-hand domain. -
Cellular localization
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. - Information by UniProt
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Database links
- Entrez Gene: 71877 Mouse
- SwissProt: Q9D9T8 Mouse
- Unigene: 29178 Mouse
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Alternative names
- EF hand domain (C terminal) containing 1 antibody
- EF hand domain containing protein 1 antibody
- EF-hand domain-containing protein 1 antibody
see all
Images
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Western blot - Anti-EFHC1 antibody (ab80861)All lanes : Anti-EFHC1 antibody (ab80861) at 1 µg/ml
Lane 1 : Mouse Cortex Tissue Lysate
Lane 2 : Mouse Hippocampus Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa, 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
Datasheets and documents
References (0)
ab80861 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-EFHC1 antibody (ab80861)All lanes : Anti-EFHC1 antibody (ab80861) at 1 µg/ml
Lane 1 : Mouse Cortex Tissue Lysate
Lane 2 : Mouse Hippocampus Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 75 kDa
Observed band size: 77 kDa why is the actual band size different from the predicted?
Additional bands at: 150 kDa, 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
