Anti-EDG6/S1PR4 antibody (ab92993)
Key features and details
- Rabbit polyclonal to EDG6/S1PR4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EDG6/S1PR4 antibody
See all EDG6/S1PR4 primary antibodies -
Description
Rabbit polyclonal to EDG6/S1PR4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human EDG6/S1PR4 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab126638) -
Positive control
- This antibody gave a positive signal in Jurkat whole cell lysate as well as the following tissue lysates: Human Spleen; Human Tonsil; Human Lymph node.
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General notes
This product was previously labelled as EDG6
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications CowDogPigMacaque monkeyGorillaApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 42 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 42 kDa).Target
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Function
Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. May be involved in cell migration processes that are specific for lymphocytes. -
Tissue specificity
Specifically expressed in fetal and adult lymphoid and hematopoietic tissue as well as in lung. Considerable level of expression in adult and fetal spleen as well as adult peripheral leukocytes and lung. Lower expression in adult thymus, lymph node, bone marrow, and appendix as well as in fetal liver, thymus, and lung. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 539534 Cow
- Entrez Gene: 101150342 Gorilla
- Entrez Gene: 8698 Human
- Entrez Gene: 100517997 Pig
- Omim: 603751 Human
- SwissProt: O95977 Human
- Unigene: 662006 Human
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Alternative names
- EDG6 antibody
- Endothelial differentiation lysophosphatidic acid G protein coupled receptor 6 antibody
- Endothelial differentiation G protein coupled receptor 6 antibody
see all
Images
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Western blot - Anti-EDG6/S1PR4 antibody (ab92993)All lanes : Anti-EDG6/S1PR4 antibody (ab92993) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : Tonsil (Human) Tissue Lysate
Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutesEDG6/S1PR4 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Datasheets and documents
References (0)
ab92993 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-EDG6/S1PR4 antibody (ab92993)All lanes : Anti-EDG6/S1PR4 antibody (ab92993) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : Tonsil (Human) Tissue Lysate
Lane 3 : Human lymph node tissue lysate - total protein (ab29871)
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Additional bands at: 56 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutesEDG6/S1PR4 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
