Anti-ECE1 antibody (ab279576)
Key features and details
- Rabbit polyclonal to ECE1
- Suitable for: ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ECE1 antibody -
Description
Rabbit polyclonal to ECE1 -
Host species
Rabbit -
Specificity
Human ECE1/ECE-1
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Tested applications
Suitable for: ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human ECE1 aa 50-800 (extracellular). Extracellular domain of human ECE1 isoform B was expressed, with a polyhistidine tag at the N-terminus.
Database link: P42892-1 -
Positive control
- ICC: HepG2 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: 100% PBS -
Concentration information loading... -
Purification notes
Protein A & Antigen Affinity chromatography -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-ECE1 antibody (ab279576)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.3% Triton X-100 permeabilized and 10% serum blocked HepG2 cells stained for ECE1 using ab279576 at 1/1000 dilution at 4℃ overnight, followed by Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.
Secondary antibody only control: Goat Anti-Rabbit IgG (Alexa Fluor® 488).