Anti-E2F1 antibody (ab112580)
Key features and details
- Rabbit polyclonal to E2F1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-E2F1 antibody
See all E2F1 primary antibodies -
Description
Rabbit polyclonal to E2F1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa whole cell lysate and nuclear lysate. Mouse liver tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab112580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). Application notesIs unsuitable for ICC/IF.Target
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Function
Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. -
Sequence similarities
Belongs to the E2F/DP family. -
Post-translational
modificationsPhosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 535369 Cow
- Entrez Gene: 1869 Human
- Entrez Gene: 13555 Mouse
- Omim: 189971 Human
- SwissProt: Q01094 Human
- SwissProt: Q61501 Mouse
- Unigene: 654393 Human
- Unigene: 18036 Mouse
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Alternative names
- Dmel\CG6376 antibody
- Dmel_CG6376 antibody
- drosE2F1 antibody
see all
Images
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Western blot - Anti-E2F1 antibody (ab112580)Anti-E2F1 antibody (ab112580) at 1 µg/ml + Mouse liver tissue lysate at 20 µg
Secondary
Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (1:10000 dilution) at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking buffer: 2% BSA
Gel type: MOPS
Loading control: GAPDH (ab8245 @ 0.1 ug/ml). Secondary: Goat anti-Mouse IgG H&L (IRDye® 680RD)
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Western blot - Anti-E2F1 antibody (ab112580)Lane 1 : Anti-E2F1 antibody (ab112580) at 1 µg
Lanes 2-3 : Anti-E2F1 antibody (ab112580) at 1 µg/ml
Lane 1 : HeLa whole cell lysate at 20 µg
Lane 2 : HeLa nuclear lysate at 20 µg
Lane 3 : HeLa whole cell lysate - Nocodozole Stimulated
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking buffer: 2% BSA
Gel type: MOPS
Loading control: GAPDH (ab8245 @ 0.1 ug/ml). Secondary: Goat anti-Mouse IgG H&L (IRDye® 680RD)
Protocols
Datasheets and documents
References (5)
ab112580 has been referenced in 5 publications.
- Qiu L et al. Pro-Angiogenic and Pro-Inflammatory Regulation by lncRNA MCM3AP-AS1-Mediated Upregulation of DPP4 in Clear Cell Renal Cell Carcinoma. Front Oncol 10:705 (2020). PubMed: 32714856
- Bervoets S et al. Transcriptional dysregulation by a nucleus-localized aminoacyl-tRNA synthetase associated with Charcot-Marie-Tooth neuropathy. Nat Commun 10:5045 (2019). PubMed: 31695036
- Zhao YX et al. microRNA-372 inhibits proliferation and induces apoptosis in human breast cancer cells by directly targeting E2F1. Mol Med Rep 16:8069-8075 (2017). PubMed: 28944922
- Scott MC et al. Aberrant Retinoblastoma (RB)-E2F Transcriptional Regulation Defines Molecular Phenotypes of Osteosarcoma. J Biol Chem 290:28070-83 (2015). PubMed: 26378234
- Marathe S et al. Notch signaling in response to excitotoxicity induces neurodegeneration via erroneous cell cycle reentry. Cell Death Differ 22:1775-84 (2015). PubMed: 25822340
Images
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Western blot - Anti-E2F1 antibody (ab112580)Anti-E2F1 antibody (ab112580) at 1 µg/ml + Mouse liver tissue lysate at 20 µg
Secondary
Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (1:10000 dilution) at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking buffer: 2% BSA
Gel type: MOPS
Loading control: GAPDH (ab8245 @ 0.1 ug/ml). Secondary: Goat anti-Mouse IgG H&L (IRDye® 680RD)
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Western blot - Anti-E2F1 antibody (ab112580)Lane 1 : Anti-E2F1 antibody (ab112580) at 1 µg
Lanes 2-3 : Anti-E2F1 antibody (ab112580) at 1 µg/ml
Lane 1 : HeLa whole cell lysate at 20 µg
Lane 2 : HeLa nuclear lysate at 20 µg
Lane 3 : HeLa whole cell lysate - Nocodozole Stimulated
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?Blocking buffer: 2% BSA
Gel type: MOPS
Loading control: GAPDH (ab8245 @ 0.1 ug/ml). Secondary: Goat anti-Mouse IgG H&L (IRDye® 680RD)