Anti-DTX3L antibody (ab70621)
Key features and details
- Rabbit polyclonal to DTX3L
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DTX3L antibody
See all DTX3L primary antibodies -
Description
Rabbit polyclonal to DTX3L -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residue 700 and the C-terminus (residue 740) of human DTX3L (NP_612144.1)
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Positive control
- Whole cell lysates from HeLa, 293T and NIH3T3 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DTX3L antibody (ab70621)All lanes : Anti-DTX3L antibody (ab70621) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 83 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa, 40 kDa. We are unsure as to the identity of these extra bands.
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Immunoprecipitation - Anti-DTX3L antibody (ab70621)Immunoprecipitation of HeLa whole cell lysate (1mg) using ab70621 at 3 µg/mg lysate (lane 1) or control IgG (lane 2). 20% of the immunoprecipitate was analysed by Western blot using ab70621 at 1 µg/ml.
