Anti-Zfp57 antibody (ab45341)
Key features and details
- Rabbit polyclonal to Zfp57
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Zfp57 antibody -
Description
Rabbit polyclonal to Zfp57 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide corresponding to Mouse Zfp57 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab45340)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab45341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB MouseAll applications RatApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 48 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 48 kDa).Target
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Relevance
Zfp57 is a putative transcription factor that is a marker of mouse embryonic stem cell pluripotency, the expression of which decreases in the absence of LIF. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 22715 Mouse
- Entrez Gene: 361783 Rat
- SwissProt: Q8C6P8 Mouse
- SwissProt: A0JPK3 Rat
- Unigene: 305561 Mouse
- Unigene: 106247 Rat
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Alternative names
- bA145L22 antibody
- bA145L22.2 antibody
- C6orf40 antibody
see all
Images
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Western blot - Anti-Zfp57 antibody (ab45341)Image courtesy of Dr Nozomi Takahashi, Ferguson-Smith Lab, University of Cambridge.All lanes : Anti-Zfp57 antibody (ab45341) at 1/1000 dilution
Lanes 1-2 : Wild type mouse embryonic stem cell lysate
Lane 3 : Zfp57 knockout mouse embryonic stem cell lysate
Secondary
All lanes : Goat anti Rabbit HRP at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 48 kDa
Datasheets and documents
References (10)
ab45341 has been referenced in 10 publications.
- Matsuzaki H et al. Synthetic DNA fragments bearing ICR cis elements become differentially methylated and recapitulate genomic imprinting in transgenic mice. Epigenetics Chromatin 11:36 (2018). PubMed: 29958543
- Coluccio A et al. Individual retrotransposon integrants are differentially controlled by KZFP/KAP1-dependent histone methylation, DNA methylation and TET-mediated hydroxymethylation in naïve embryonic stem cells. Epigenetics Chromatin 11:7 (2018). PubMed: 29482634
- Fu J et al. Gene expression profiles of glomerular endothelial cells support their role in the glomerulopathy of diabetic mice. Kidney Int 94:326-345 (2018). PubMed: 29861058
- Lee CC et al. The Role of N-a-acetyltransferase 10 Protein in DNA Methylation and Genomic Imprinting. Mol Cell 68:89-103.e7 (2017). PubMed: 28943313
- Anvar Z et al. ZFP57 recognizes multiple and closely spaced sequence motif variants to maintain repressive epigenetic marks in mouse embryonic stem cells. Nucleic Acids Res 44:1118-32 (2016). ChIP . PubMed: 26481358
- Luo Z et al. Regulation of the imprinted Dlk1-Dio3 locus by allele-specific enhancer activity. Genes Dev 30:92-101 (2016). ChIP-sequencing ; Mouse . PubMed: 26728555
- Riso V et al. ZFP57 maintains the parent-of-origin-specific expression of the imprinted genes and differentially affects non-imprinted targets in mouse embryonic stem cells. Nucleic Acids Res 44:8165-78 (2016). PubMed: 27257070
- Strogantsev R et al. Allele-specific binding of ZFP57 in the epigenetic regulation of imprinted and non-imprinted monoallelic expression. Genome Biol 16:112 (2015). WB, ChIP . PubMed: 26025256
- Baglivo I et al. Genetic and epigenetic mutations affect the DNA binding capability of human ZFP57 in transient neonatal diabetes type 1. FEBS Lett 587:1474-81 (2013). ChIP ; Mouse . PubMed: 23499433
- Quenneville S et al. In Embryonic Stem Cells, ZFP57/KAP1 Recognize a Methylated Hexanucleotide to Affect Chromatin and DNA Methylation of Imprinting Control Regions. Mol Cell 44:361-72 (2011). WB ; Mouse . PubMed: 22055183
Images
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Western blot - Anti-Zfp57 antibody (ab45341) Image courtesy of Dr Nozomi Takahashi, Ferguson-Smith Lab, University of Cambridge.All lanes : Anti-Zfp57 antibody (ab45341) at 1/1000 dilution
Lanes 1-2 : Wild type mouse embryonic stem cell lysate
Lane 3 : Zfp57 knockout mouse embryonic stem cell lysate
Secondary
All lanes : Goat anti Rabbit HRP at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 48 kDa