Anti-ds DNA antibody [35I9 DNA] (ab27156)
![Anti-ds DNA antibody [35I9 DNA] (ab27156)](https://www.abcam.com/ps/products/27/ab27156/Images/ab27156-448957-antidsdnaantibodyimmunohistochemistryskinhuman.jpg "Anti-ds DNA antibody [35I9 DNA] (ab27156)")
Key features and details
- Mouse monoclonal [35I9 DNA] to ds DNA
- Suitable for: IHC-P
- Isotype: IgG2a
Overview
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Product name
Anti-ds DNA antibody [35I9 DNA]
See all ds DNA primary antibodies -
Description
Mouse monoclonal [35I9 DNA] to ds DNA -
Host species
Mouse -
Specificity
Primarily Double stranded DNA. Measurements by immuno-CE yielded KD's of 0.71 µM and 0.09 µM, for the interaction of this antibody with ss- and dsDNA, respectively. Strong reactivity with both ss- and dsDNA has been observed on dotblots as well as very weak reactivity with RNA. -
Tested applications
Suitable for: IHC-Pmore details -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- ICC/IF: Stylonychia lemnae cells; Drosophila melanogaster larval brain cells; MEFs. ELISA: HEK293 cell lysate. IHC-P: human skin tissue sections
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General notes
This antibody has been shown to be useful, in the detection of dsDNA in fx. Crithidia luciliae -a monoflagellate protozoan,containing a giant mitochondrion. Very good reactivity both with dsDNA and ssDNA on NC-dotblots has been observed. The minimal size for DNA binding for this antibody is >16 bases and there is a inverse proportionality between binding and ionic strength.
Abcam recommended secondaries - Goat Anti-Mouse HRP (ab205719) and Goat Anti-Mouse Alexa Fluor® 488 (ab150113).
See other anti-mouse secondary antibodies that can be used with this antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Primary antibody notes
This antibody has been shown to be useful, using immunofluorescence, in the detection of dsDNA in fx. Crithidia luciliae -a monoflagellate protozoan,containing a giant mitochondrion. The antibody can be used for easy quantification of in vitro cell proliferation by ELISA and has successfully been used for monitoring cytotoxity and apoptosis as well as ELISA-based co-culture angiogenesis and proliferation assays. Very good reactivity both with dsDNA and ssDNA on NC-dotblots has been observed. The minimal size for DNA binding for this antibody is >16 bases and there is a inverse proportionality between binding and ionic strength. -
Clonality
Monoclonal -
Clone number
35I9 DNA -
Myeloma
x63-Ag8.653 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ds DNA antibody [35I9 DNA] (ab27156)
IHC image of ds DNA staining in a section of formalin-fixed paraffin-embedded normal human skin* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab27156, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
