Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DPEP3 antibody [EPR21862] - BSA and Azide free (ab238646)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21862] to DPEP3 - BSA and Azide free
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-DPEP3 antibody [EPR21862] - BSA and Azide free
    See all DPEP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR21862] to DPEP3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human testis tissue.

  • General notes

    Ab238646 is the carrier-free version of ab235451. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238646 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-DPEP3 antibody [EPR21862] - BSA and Azide free (ab238646)
    Immunoprecipitation - Anti-DPEP3 antibody [EPR21862] - BSA and Azide free (ab238646)

    DPEP3 was immunoprecipitated from 0.35 mg of human testis lysate with ab235451 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab235451 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Human testis lysate 10 μg (Input).
    Lane 2: ab235451 IP in human testis lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab235451 in human testis lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235451).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP3 antibody [EPR21862] - BSA and Azide free (ab238646)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP3 antibody [EPR21862] - BSA and Azide free (ab238646)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling DPEP3 with ab235451 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on tumor epithelial cells of one case human breast cancer is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Please note that DPEP3 was not detected in every breast cancer tissue sample tested due to the heterogeneity of expression.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235451).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP3 antibody [EPR21862] - BSA and Azide free (ab238646)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP3 antibody [EPR21862] - BSA and Azide free (ab238646)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling DPEP3 with ab235451 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and cytoplasmic staining on human germ cells but not Leydig cells (arrow) or Sertoli cells (arrowhead) (PMID: 21724266) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab235451).

     

  • Anti-DPEP3 antibody [EPR21862] - BSA and Azide free (ab238646)
    Anti-DPEP3 antibody [EPR21862] - BSA and Azide free (ab238646)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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