Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DPEP3 antibody [EPR21862] (ab235451)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21862] to DPEP3
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-DPEP3 antibody [EPR21862]
    See all DPEP3 primary antibodies

  • Description

    Rabbit monoclonal [EPR21862] to DPEP3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human testis lysate. IP: Human testis lysate. IHC-P: Human testis and breast cancer tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP3 antibody [EPR21862] (ab235451)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP3 antibody [EPR21862] (ab235451)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling DPEP3 with ab235451 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining on tumor epithelial cells of one case human breast cancer is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Please note that DPEP3 was not detected in every breast cancer tissue sample tested due to the heterogeneity of expression.

  • Western blot - Anti-DPEP3 antibody [EPR21862] (ab235451)
    Western blot - Anti-DPEP3 antibody [EPR21862] (ab235451)
    Anti-DPEP3 antibody [EPR21862] (ab235451) at 1/1000 dilution + Human testis lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 54 kDa
    Observed band size: 75 kDa
    why is the actual band size different from the predicted?


    Exposure time: 48 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-DPEP3 antibody [EPR21862] (ab235451)
    Immunoprecipitation - Anti-DPEP3 antibody [EPR21862] (ab235451)

    DPEP3 was immunoprecipitated from 0.35 mg of human testis lysate with ab235451 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab235451 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    Lane 1: Human testis lysate 10 μg (Input).
    Lane 2: ab235451 IP in human testis lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab235451 in human testis lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP3 antibody [EPR21862] (ab235451)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DPEP3 antibody [EPR21862] (ab235451)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling DPEP3 with ab235451 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous and cytoplasmic staining on human germ cells but not Leydig cells (arrow) or Sertoli cells (arrowhead) (PMID: 21724266) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-DPEP3 antibody [EPR21862] (ab235451)
    Anti-DPEP3 antibody [EPR21862] (ab235451)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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