Anti-Dopamine antibody (ab6427)
Key features and details
- Rabbit polyclonal to Dopamine
- Suitable for: ICC, ICC/IF, ELISA
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Dopamine antibody
See all Dopamine primary antibodies -
Description
Rabbit polyclonal to Dopamine -
Host species
Rabbit -
Tested applications
Suitable for: ICC, ICC/IF, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule by a Glutaraldehyde linker.
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General notes
The antiserum was tested using the free floating PAP technique on rat dopaminergic areas. The anti-conjugated Dopamine antibodies gave good staining between 1/2000-1/5000 dilution in these areas.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Primary antibody notes
The antiserum was tested using the free floating PAP technique on rat dopaminergic areas. The anti-conjugated Dopamine antibodies gave good staining between 1/2000-1/5000 dilution in these areas. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-Dopamine antibody (ab6427) This image is courtesy of an anonymous Abreviewab6427 at 1/5000 staining human stem cells (differentiated to neurons) by ICC. The cells were paraformaldehyde fixed and blocked with BSA before incubation with the antibody for 2 hours. A PE conjugated goatanti-rabbit antibody was used as the secondary.
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Immunocytochemistry/ Immunofluorescence - Anti-Dopamine antibody (ab6427)ab6427 staining dopamine in PC12 cells treated with ghrelin (rat) (ab120231), by ICC/IF. Decrease in dopamine expression correlates with increased concentration of ghrelin, as described in literature.
The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120231 (ghrelin) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab6427 (1/200 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
