Anti-DNAJB9 antibody (ab118282)
Key features and details
- Goat polyclonal to DNAJB9
- Suitable for: WB, Flow Cyt, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-DNAJB9 antibody
See all DNAJB9 primary antibodies -
Description
Goat polyclonal to DNAJB9 -
Host species
Goat -
Tested applications
Suitable for: WB, Flow Cyt, ICCmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Dog, Pig, Chimpanzee
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Immunogen
Synthetic peptide:
PDAEAKFREIAEAYE
with a Cysteine residue linker, corresponding to internal sequence amino acids 61-75 of Human DNAJB9 (NP_036460.1). -
Positive control
- WB: Human liver and kidney lysates. Mouse liver lysate; ICC: A431 and U2OS cells; Flow Cyt: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab118282 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DNAJB9 antibody (ab118282)All lanes : Anti-DNAJB9 antibody (ab118282) at 1 µg/ml
Lane 1 : Human liver lysate (in RIPA buffer)
Lane 2 : Human kidney lysate (in RIPA buffer)
Lane 3 : Mouse liver lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 26 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
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Immunocytochemistry - Anti-DNAJB9 antibody (ab118282)Immunocytochemistry analysis of paraformaldehyde fixed U2OS cells labeling DNAJB9 with ab118282. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
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Immunocytochemistry - Anti-DNAJB9 antibody (ab118282)Immunocytochemistry analysis of paraformaldehyde fixed A431 cells labeling DNAJB9 with ab118282. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
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Flow Cytometry - Anti-DNAJB9 antibody (ab118282)Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line) labeling DNAJB9 with ab118282. Cells permeabilized with 0.5% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
