Antibodies, Proteins, Kits and Reagents for Life Science

Anti-DMGDH antibody [EPR16908] - BSA and Azide free (ab251253)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16908] to DMGDH - BSA and Azide free
Suitable for: WB, IP, IHC-P
Reacts with: Human

Product name

Anti-DMGDH antibody [EPR16908] - BSA and Azide free
See all DMGDH primary antibodies
Description

Rabbit monoclonal [EPR16908] to DMGDH - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, IP, IHC-Pmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
ab251253 is the carrier-free version of ab198292.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR16908
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMGDH antibody [EPR16908] - BSA and Azide free (ab251253)

This data was developed using ab198292, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DMGDH with ab198292 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. Inset image: negative control obtained using PBS instead of ab198292 and secondary antibody only.
Note: Cytoplasm staining on human kidney tissue was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-DMGDH antibody [EPR16908] - BSA and Azide free (ab251253)

All lanes : Anti-DMGDH antibody [EPR16908] (ab198292) at 1/10000 dilution

Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human fetal liver tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 97 kDa

Exposure time: 2 seconds

This data was developed using ab198292, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Western blot - Anti-DMGDH antibody [EPR16908] - BSA and Azide free (ab251253)

Anti-DMGDH antibody [EPR16908] (ab198292) at 1/1000 dilution + HepG2 (Human liver hepatocellular carcinoma) cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Developed using the ECL technique.

Predicted band size: 97 kDa

Exposure time: 3 minutes

This data was developed using ab198292, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DMGDH antibody [EPR16908] - BSA and Azide free (ab251253)

This data was developed using ab198292, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling DMGDH with ab198292 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. Inset image: negative control obtained using PBS instead of ab198292 and secondary antibody only.
Note: Cytoplasm staining on human liver tissue was observed. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-DMGDH antibody [EPR16908] - BSA and Azide free (ab251253)

This data was developed using ab198292, the same antibody clone in a different buffer formulation.

DMGDH protein was immunoprecipitation from 1mg of Human fetal liver whole cell lysate with ab198292 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab198292 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1000 dilution. Lane 1: Input, Human fetal liver whole cell lysate, 10ug. Lane 2: IP of DMGDH from Human fetal liver whole cell lysate. Lane 3: IP using Rabbit monoclonal IgG (ab172730) instead of ab198292 in Human fetal liver whole cell lysate.

Blocking and dilution buffer: 5% NFDM/TBST.
Anti-DMGDH antibody [EPR16908] - BSA and Azide free (ab251253)

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