Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DLX3 antibody [NCI-R183-14] - BSA and Azide free (ab251528)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [NCI-R183-14] to DLX3 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-DLX3 antibody [NCI-R183-14] - BSA and Azide free
    See all DLX3 primary antibodies

  • Description

    Rabbit monoclonal [NCI-R183-14] to DLX3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251528 is the carrier-free version of ab211492. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251528 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-DLX3 antibody [NCI-R183-14] - BSA and Azide free (ab251528)
    Western blot - Anti-DLX3 antibody [NCI-R183-14] - BSA and Azide free (ab251528) The data was kindly provided by Dr. Maria Morasso (NIH/NIAMS).
    All lanes : Anti-DLX3 antibody [NCI-R183-14] (ab211492) at 1/1000 dilution

    Lane 1 : Saos-2 (Human osteosarcoma cell line) cell transfected with mouse TetOFFv5DLX3/flag TDO treated with DOX(Doxorubicin 2µg/ml)
    Lane 2 : Saos-2 (Human osteosarcoma cell line) cell transfected with mouse TetOFFv5DLX3/flag TDO untreated with DOX(Doxorubicin 2µg/ml)
    Lane 3 : Saos-2 (Human osteosarcoma cell line) cell transfected with human TetOFFv5DLX3/flag TDO treated with DOX(Doxorubicin 2µg/ml)
    Lane 4 : Saos-2 (Human osteosarcoma cell line) cell transfected with human TetOFFv5DLX3/flag TDO untreated with DOX(Doxorubicin 2µg/ml)

    Predicted band size: 32 kDa
    Observed band size: 38,45 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    This data was developed using ab211492, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    This product only recognizes DLX3 carrying the TDO (Tricho-Dento-Osseous syndrome) frameshift mutation.

  • Anti-DLX3 antibody [NCI-R183-14] - BSA and Azide free (ab251528)
    Anti-DLX3 antibody [NCI-R183-14] - BSA and Azide free (ab251528)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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