Anti-DLX-3 antibody [Anti-Dlx3b] (ab174436)
![Anti-DLX-3 antibody [Anti-Dlx3b] (ab174436)](https://www.abcam.com/ps/products/174/ab174436/Images/ab174436-201978-anti-dlx-3-antibody-anti-dlx3b-ihc-wholemount.jpg "Anti-DLX-3 antibody [Anti-Dlx3b] (ab174436)")
Key features and details
- Mouse monoclonal [Anti-Dlx3b] to DLX-3
- Suitable for: IHC - Wholemount
- Reacts with: Zebrafish
- Isotype: IgG1
Overview
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Product name
Anti-DLX-3 antibody [Anti-Dlx3b] -
Description
Mouse monoclonal [Anti-Dlx3b] to DLX-3 -
Host species
Mouse -
Tested applications
Suitable for: IHC - Wholemountmore details -
Species reactivity
Reacts with: Zebrafish -
Immunogen
Recombinant full length protein. This information is considered to be commercially sensitive.
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Positive control
- IHC-Wholemount: 19 somite zebrafish.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Purity
SDS-PAGE -
Purification notes
ab174436 is produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation. Near homogeneity as judged by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
Anti-Dlx3b -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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IHC - Wholemount - Anti-DLX-3 antibody [Anti-Dlx3b] (ab174436)
ab174436 staining DLX-3 in 19 somite zebrafish by IHC-wholemount. The embryo was fixed with 4% PFA at 4°C over night and an antigen retrieval step was performed with Tris-HCl pH 9. Blocking of the sample was done with 10% sheep serum in PBS containing 01% Tween 20 and 0.5% Triton X, for 60 minutes at 23°C, followed by staining with ab174436 at 1/100 in blocking solution for 16h at 4°C. An alexa 488 conjugated goat anti-mouse polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei are stained in blue with DAPI. The forebrain is stained by Dlx3b (in green).
