Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22592-18] to DLL3 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free
    See all DLL3 primary antibodies

  • Description

    Rabbit monoclonal [EPR22592-18] to DLL3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with DLL3 (WT) expression vector containing a myc-His-tag®, whole cell lysate. IHC-P: Human small cell lung cancer, cerebrum and glioma tissue; Mouse embryonic brain of day 14.5 tissue. ICC/IF: HEK-293T cells. Flow: HEK-293T cells. IP: HEK-293T transfected with DLL3 expression construct containing a myc-His-tag® whole cell lysate.

  • General notes

    Ab255694 is the carrier-free version of ab229902. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255694 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)
    Flow Cytometry - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with DLL3 expression construct containing a myc-His-tag®  labelling DLL3 with ab229902 at 1/600 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730, Left) isotype control. 

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or ab229902 (Right). Then stained with anti-myc-tag conjugated to Alexa Fluor® 647.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229902).

  • Immunocytochemistry/ Immunofluorescence - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)
    Immunocytochemistry/ Immunofluorescence - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells labelling DLL3 with ab229902 at 1/100 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary antibody at 1/500 dilution (Green). An anti-myc-tag mAb (Alexa Fluor® 488 Conjugate) was used at 1/100 dilution. Confocal image showing positive staining in HEK-293T cells transfected with DLL3 expression construct containing a myc-His-tag® is observed.  The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229902).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)

    Immunohistochemical analysis of paraffin-embedded human small cell lung cancer tissue labeling DLL3 with ab229902 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human small cell lung cancer (PMID: 28487384, 30397180) is observed.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    The section was incubated with ab229902 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229902).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)

    Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling DLL3 with ab229902 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human glioma (PMID: 30397180) is observed.

    The section was incubated with ab229902 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229902).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)

    Immunohistochemical analysis of paraffin-embedded mouse embryonic brain of day 14.5 tissue labeling DLL3 with ab229902 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse embryonic brain of day 14.5 (PMID: 19389376) is observed.

    The section was incubated with ab229902 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229902).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling DLL3 with ab229902 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: no staining on human cerebrum (PMID: 30397180) is observed.

    The section was incubated with ab229902 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229902).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling DLL3 with ab229902 at 1/100 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: no staining on mouse cerebrum (PMID: 30397180) is observed.

    The section was incubated with ab229902 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229902).

  • Immunoprecipitation - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)
    Immunoprecipitation - Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)

    DLL3 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) transfected with DLL3 expression construct containing a myc-His-tag®  whole cell lysate with ab229902 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab229902 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

    Lane 1: HEK-293T (human embryonic kidney epithelial cell) transfected with DLL3 expression construct containing a myc-His-tag®  whole cell lysate 10μg

    Lane 2: ab229902 IP in HEK-293T transfected with DLL3 expression construct containing a myc-His-tag®  whole cell lysate (Input).

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229902 in HEK-293T transfected with DLL3 expression construct containing a myc-His-tag®  whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds. 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229902).

  • Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)
    Anti-DLL3 antibody [EPR22592-18] - BSA and Azide free (ab255694)

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