Anti-DCTN5 antibody [EPR11253] (ab157197)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11253] to DCTN5
- Suitable for: WB, IHC-P, ICC/IF, IP
- Reacts with: Human
Overview
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Product name
Anti-DCTN5 antibody [EPR11253]
See all DCTN5 primary antibodies -
Description
Rabbit monoclonal [EPR11253] to DCTN5 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanIP HumanWB Human -
Immunogen
Recombinant fragment corresponding to Human DCTN5.
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Positive control
- MCF7, Human fetal liver, and SH-SY5Y lysates; Human kidney tissue; MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11253 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-DCTN5 antibody [EPR11253] (ab157197) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : Human fetal liver cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 20 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN5 antibody [EPR11253] (ab157197)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DCTN5 with ab157197 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunofluorescent analysis of MCF7 cells labeling DCTN5 with ab157197 at 1/250 dilution.
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Detection of DCTN5 by Western Blot of Immunprecipitate. MCF7 cell lysate immunoprecipitated using ab156197 at 1/10 dilution.
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