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Immunology Adaptive Immunity T Cells CD

Anti-DC-SIGN antibody [5C2G9] (ab233749)

Anti-DC-SIGN antibody [5C2G9] (ab233749)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [5C2G9] to DC-SIGN
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-DC-SIGN antibody [5C2G9]
    See all DC-SIGN primary antibodies
  • Description

    Mouse monoclonal [5C2G9] to DC-SIGN
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Recombinant fragment corresponding to Human DC-SIGN aa 250 to the C-terminus. (Expressed in E.coli).
    Database link: Q9NNX6

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: THP-1, HL-60 and A431 cell lysates; Human DC-SIGN (aa 270-404) recombinant protein; DC-SIGN (aa 270-404)-hIgGFc transfected HEK-293 cell lysate. Flow Cyt: Ramos cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified fom tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    5C2G9
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • T Cell Adhesion
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV-related

Images

  • Western blot - Anti-DC-SIGN antibody [5C2G9] (ab233749)
    Western blot - Anti-DC-SIGN antibody [5C2G9] (ab233749)
    Anti-DC-SIGN antibody [5C2G9] (ab233749) at 1/500 dilution + Human DC-SIGN (aa 270-404) recombinant protein

    Predicted band size: 46 kDa

  • Western blot - Anti-DC-SIGN antibody [5C2G9] (ab233749)
    Western blot - Anti-DC-SIGN antibody [5C2G9] (ab233749)
    All lanes : Anti-DC-SIGN antibody [5C2G9] (ab233749) at 1/500 dilution

    Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : DC-SIGN (aa 270-404)-hIgGFc transfected HEK-293 cell lysate

    Predicted band size: 46 kDa

  • Western blot - Anti-DC-SIGN antibody [5C2G9] (ab233749)
    Western blot - Anti-DC-SIGN antibody [5C2G9] (ab233749)
    All lanes : Anti-DC-SIGN antibody [5C2G9] (ab233749) at 1/500 dilution

    Lane 1 : THP-1 (human monocytic leukemia cell line) cell lysate
    Lane 2 : HL-60 (human promyelocytic leukemia cell line) cell lysate
    Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate

    Predicted band size: 46 kDa

  • Flow Cytometry - Anti-DC-SIGN antibody [5C2G9] (ab233749)
    Flow Cytometry - Anti-DC-SIGN antibody [5C2G9] (ab233749)

    Flow cytometric analysis of Ramos (human Burkitt's lymphoma cell line) cells labeling DC-SIGN with ab233749 at 1/200 dilution (green) compared with a negative control (red).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-DC-SIGN antibody [5C2G9] (ab233749)

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