Anti-DAP3 antibody (ab2637)
Key features and details
- Goat polyclonal to DAP3
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DAP3 antibody
See all DAP3 primary antibodies -
Description
Goat polyclonal to DAP3 -
Host species
Goat -
Specificity
This antibody is expected to recognise both human isoforms of this protein. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human DAP3 aa 387-398 (C terminal).
Sequence:NPSLLERHCAYL
(Peptide available asab22922) -
Positive control
- WB: HeLa and HepG2 cell lysate and human kidney tissue lysate. ICC/IF: MCF7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DAP3 antibody (ab2637)Anti-DAP3 antibody (ab2637) at 0.3 µg/ml + Human kidney tissue lysate in RIPA buffer at 30 µg
Predicted band size: 46 kDaDetected using chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-DAP3 antibody (ab2637)Immunocytochemistry/Immunofluorescence analysis of MCF7 cells labelling DAP3 with ab2637 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.
Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).
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Western blot - Anti-DAP3 antibody (ab2637)All lanes : Anti-DAP3 antibody (ab2637) at 0.3 µg/ml
Lane 1 : HeLa cell lysate in RIPA buffer
Lane 2 : HepG2 cell lysate in RIPA buffer
Lysates/proteins at 30 µg per lane.
Predicted band size: 46 kDaDetected using chemiluminescence.
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Western blot - Anti-DAP3 antibody (ab2637)All lanes : Anti-DAP3 antibody (ab2637) at 1 µg/ml (for 1 hour)
Lane 1 : HeLa cell lysate in RIPA buffer - control
Lane 2 : HeLa cell lysate in RIPA buffer - after siRNA mediated DAP3 knock-down expression
Lysates/proteins at 35 µg per lane.
Predicted band size: 46 kDaLevel of knock-down relative to actin expression level determined by RT-PCR.
