Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15867] to DAP13/NDUFA12 - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free
    See all DAP13/NDUFA12 primary antibodies

  • Description

    Rabbit monoclonal [EPR15867] to DAP13/NDUFA12 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251142 is the carrier-free version of ab192607 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab251142 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as DAP13

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)
    Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)
    Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607) at 1/1000 dilution + Human skeletal muscle at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 17,7 kDa



    This data was developed using ab192607, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)
    Immunocytochemistry - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)

    This data was developed using ab192607, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of Hela cells permeabilised in 0.1% Triton X-100 and fixed in 4% paraformaldehyde labeling DAP13/NDUFA12 with ab192607 at 1/50 (5.9 μg/ml) and Goat anti rabbit IgG(Alexa Fluor® 488) ab150077. Counterstained with DAPI.
  • Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)
    Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)
    All lanes : Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607) at 1/5000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17,7 kDa
    Additional bands at: 7 kDa (possible isoform)



    This data was developed using ab192607, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)
    Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)
    Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607) at 1/5000 dilution + Rat muscle tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17,7 kDa



    This data was developed using ab192607, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)
    Western blot - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)
    All lanes : Anti-DAP13/NDUFA12 antibody [EPR15867] (ab192607) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17,7 kDa



    This data was developed using ab192607, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)
    This data was developed using ab192607, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin fixed paraffin embedded Human pancreas labeling DAP13/NDUFA12 with ab192607 at 1/600 (0.5μg/ml) dilution and prediluted HRP polymer to Rabbit/Mouse IgG as secondary. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)
    This data was developed using ab192607, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin fixed paraffin embedded mouse liver labeling DAP13/NDUFA12 with ab192607 at 1/600 (0.5μg/ml) dilution and prediluted HRP polymer to Rabbit/Mouse IgG as secondary. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)
    Anti-DAP13/NDUFA12 antibody [EPR15867] - BSA and Azide free (ab251142)

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