Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 7 antibody [SP52] (ab183344)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP52] to Cytokeratin 7
  • Suitable for: ICC, IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Cytokeratin 7 antibody [SP52]
    See all Cytokeratin 7 primary antibodies

  • Description

    Rabbit monoclonal [SP52] to Cytokeratin 7

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human breast carcinoma, breast, pancreatic adenocarcinoma, breast ductal cell carcinoma, bladder, bladder transitional cell carcinoma, prostate, prostate adenocarcinoma, endometrium, endometrial adenocarcinoma and pancreas tissues; WB: HeLa cell and A549 cell lysates; ICC: HeLa cells.

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, 98% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    SP52

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Cytokeratin 7 antibody [SP52] (ab183344)
    Western blot - Anti-Cytokeratin 7 antibody [SP52] (ab183344)
    All lanes : Anti-Cytokeratin 7 antibody [SP52] (ab183344) at 1/25 dilution

    Lane 1 : Wild-type A549 whole cell lysate
    Lane 2 : KRT7 knockout A549 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : MCF7 whole cell lysate

    Predicted band size: 51 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab183344 observed at 50 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab183344 was shown to specifically react with KRT7 (Cytokeratin 7) in wild-type A549 cells as signal was lost in KRT7 knockout cells. Wild-type and KRT7 knockout samples were subjected to SDS-PAGE. Ab183344 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/25 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry - Anti-Cytokeratin 7 antibody [SP52] (ab183344)
    Immunocytochemistry - Anti-Cytokeratin 7 antibody [SP52] (ab183344)

    Immunocytochemistry analysis of HeLa (human cervix adenocarcinoma epithelial cell) and MCF7 (human breast adenocarcinoma epithelial cell) cells labeling Cytokeratin 7 with purified ab183344 at 1/100 (0.92 µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)

    Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.

  • Western blot - Anti-Cytokeratin 7 antibody [SP52] (ab183344)
    Western blot - Anti-Cytokeratin 7 antibody [SP52] (ab183344)
    Anti-Cytokeratin 7 antibody [SP52] (ab183344) at 1/25 dilution + HeLa cell lysate

    Predicted band size: 51 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)

    Immunohistochemical analysis of paraffin embedded Human breast tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)

    Immunohistochemical analysis of paraffin embedded Human pancreatic adenocarcinoma tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)

    Immunohistochemical analysis of paraffin embedded Human breast ductal cell carcinoma tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)

    Immunohistochemical analysis of paraffin embedded Human bladder tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)

    Immunohistochemical analysis of paraffin embedded Human bladder transitional cell carcinoma tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)

    Immunohistochemical analysis of paraffin embedded Human prostate tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)

    Immunohistochemical analysis of paraffin embedded Human prostate adenocarcinoma tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)

    Immunohistochemical analysis of paraffin embedded Human endometrium tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)

    Immunohistochemical analysis of paraffin embedded Human endometrial adenocarcinoma tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)

    Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.

  • Anti-Cytokeratin 7 antibody [SP52] (ab183344)
    Anti-Cytokeratin 7 antibody [SP52] (ab183344)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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