Anti-Cytokeratin 7 antibody [SP52] (ab183344)
![Anti-Cytokeratin 7 antibody [SP52] (ab183344)](https://www.abcam.com/ps/products/183/ab183344/Images/ab183344-345663-Anti-Cytokeratin-7-antibody-SP52-westernblot-human.jpg "Anti-Cytokeratin 7 antibody [SP52] (ab183344)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP52] to Cytokeratin 7
- Suitable for: ICC, IHC-P, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Cytokeratin 7 antibody [SP52]
See all Cytokeratin 7 primary antibodies -
Description
Rabbit monoclonal [SP52] to Cytokeratin 7 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human breast carcinoma, breast, pancreatic adenocarcinoma, breast ductal cell carcinoma, bladder, bladder transitional cell carcinoma, prostate, prostate adenocarcinoma, endometrium, endometrial adenocarcinoma and pancreas tissues; WB: HeLa cell and A549 cell lysates; ICC: HeLa cells.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, 98% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SP52 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cytokeratin 7 antibody [SP52] (ab183344)All lanes : Anti-Cytokeratin 7 antibody [SP52] (ab183344) at 1/25 dilution
Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : KRT7 knockout A549 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : MCF7 whole cell lysate
Predicted band size: 51 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab183344 observed at 50 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab183344 was shown to specifically react with KRT7 (Cytokeratin 7) in wild-type A549 cells as signal was lost in KRT7 knockout cells. Wild-type and KRT7 knockout samples were subjected to SDS-PAGE. Ab183344 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/25 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry - Anti-Cytokeratin 7 antibody [SP52] (ab183344)](https://www.abcam.com/ps/products/183/ab183344/Images/ab183344-406462-anti-cytokeratin-7-antibody-sp52-immunocytochemistry-hela-mcf7-human.jpg)
Immunocytochemistry - Anti-Cytokeratin 7 antibody [SP52] (ab183344)Immunocytochemistry analysis of HeLa (human cervix adenocarcinoma epithelial cell) and MCF7 (human breast adenocarcinoma epithelial cell) cells labeling Cytokeratin 7 with purified ab183344 at 1/100 (0.92 µg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)](https://www.abcam.com/ps/products/183/ab183344/Images/ab183344-213721-anti-cytokeratin-7-antibody-sp52-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.
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![Western blot - Anti-Cytokeratin 7 antibody [SP52] (ab183344)](https://www.abcam.com/ps/products/183/ab183344/Images/ab183344-213719-anti-cytokeratin-7-antibody-sp52-western-blot.jpg)
Western blot - Anti-Cytokeratin 7 antibody [SP52] (ab183344)Anti-Cytokeratin 7 antibody [SP52] (ab183344) at 1/25 dilution + HeLa cell lysate
Predicted band size: 51 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)](https://www.abcam.com/ps/products/183/ab183344/Images/ab183344-213722-anti-cytokeratin-7-antibody-sp52-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)Immunohistochemical analysis of paraffin embedded Human breast tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)](https://www.abcam.com/ps/products/183/ab183344/Images/ab183344-213723-anti-cytokeratin-7-antibody-sp52-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)Immunohistochemical analysis of paraffin embedded Human pancreatic adenocarcinoma tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)](https://www.abcam.com/ps/products/183/ab183344/Images/ab183344-213724-anti-cytokeratin-7-antibody-sp52-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)Immunohistochemical analysis of paraffin embedded Human breast ductal cell carcinoma tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)](https://www.abcam.com/ps/products/183/ab183344/Images/ab183344-213725-anti-cytokeratin-7-antibody-sp52-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)Immunohistochemical analysis of paraffin embedded Human bladder tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)](https://www.abcam.com/ps/products/183/ab183344/Images/ab183344-213726-anti-cytokeratin-7-antibody-sp52-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)Immunohistochemical analysis of paraffin embedded Human bladder transitional cell carcinoma tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)](https://www.abcam.com/ps/products/183/ab183344/Images/ab183344-213727-anti-cytokeratin-7-antibody-sp52-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)Immunohistochemical analysis of paraffin embedded Human prostate tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)](https://www.abcam.com/ps/products/183/ab183344/Images/ab183344-213728-anti-cytokeratin-7-antibody-sp52-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)Immunohistochemical analysis of paraffin embedded Human prostate adenocarcinoma tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)](https://www.abcam.com/ps/products/183/ab183344/Images/ab183344-213729-anti-cytokeratin-7-antibody-sp52-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)Immunohistochemical analysis of paraffin embedded Human endometrium tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)](https://www.abcam.com/ps/products/183/ab183344/Images/ab183344-213730-anti-cytokeratin-7-antibody-sp52-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)Immunohistochemical analysis of paraffin embedded Human endometrial adenocarcinoma tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)](https://www.abcam.com/ps/products/183/ab183344/Images/ab183344-213731-anti-cytokeratin-7-antibody-sp52-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SP52] (ab183344)Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling Cytokeratin 7 with ab183344 at 1/100 dilution.
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![Anti-Cytokeratin 7 antibody [SP52] (ab183344)](https://www.abcam.com/ps/products/183/ab183344/Images/ab183344-16-benefits-of-recombinant-antibodies.png)
Anti-Cytokeratin 7 antibody [SP52] (ab183344)
