Anti-Cyclin B2/CCNB2 antibody [X29.2] (ab18250)
Key features and details
- Mouse monoclonal [X29.2] to Cyclin B2/CCNB2
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Cyclin B2/CCNB2 antibody [X29.2]
See all Cyclin B2/CCNB2 primary antibodies -
Description
Mouse monoclonal [X29.2] to Cyclin B2/CCNB2 -
Host species
Mouse -
Specificity
Reacts with most cyclin Bs. -
Tested applications
Suitable for: IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Xenopus laevis Cyclin B2/CCNB2.
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General notes
This product was previously labelled as Cyclin B2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
X29.2 -
Isotype
IgG1 -
Research areas
Images
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IHC image of ab18250 staining in human cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18250, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Overlay histogram showing HeLA cells stained with ab18250 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18250, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.