Anti-Cyclin B2/CCNB2 antibody [X121.10] (ab10839)
![Anti-Cyclin B2/CCNB2 antibody [X121.10] (ab10839)](https://www.abcam.com/ps/products/10/ab10839/Images/ab10839-18596-anti-cyclin-b2-antibody-x12110-western-blot.jpg "Anti-Cyclin B2/CCNB2 antibody [X121.10] (ab10839)")
Key features and details
- Mouse monoclonal [X121.10] to Cyclin B2/CCNB2
- Suitable for: WB
- Reacts with: Xenopus laevis
- Isotype: IgG1
Overview
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Product name
Anti-Cyclin B2/CCNB2 antibody [X121.10]
See all Cyclin B2/CCNB2 primary antibodies -
Description
Mouse monoclonal [X121.10] to Cyclin B2/CCNB2 -
Host species
Mouse -
Specificity
ab10839 blots only cyclin B2/CCNB2, but cross reacts strongly with two other proteins. However, these can easily be distinguished from cyclins since they are present in interphase extracts and run well below the cyclins on SDS PAGE gels.
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Xenopus laevis -
Immunogen
Full length native protein (purified) corresponding to Xenopus laevis Cyclin B2/CCNB2.
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Epitope
The epitope for X121 lies within a region spanning the amino acids 1-112. -
Positive control
- Xenopus egg extract.
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General notes
This product was previously labelled as Cyclin B2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
X121.10 -
Myeloma
Sp2 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Cyclin B2/CCNB2 antibody [X121.10] (ab10839)All lanes : Anti-Cyclin B2/CCNB2 antibody [X121.10] (ab10839) at 1 µg/ml
Lane 1 : Interphase Xenopus egg extracts (cyclin B destroyed)
Lane 2 : Mitotic (CSF) Xenopus egg extracts (cyclin B present)
Predicted band size: 48 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa (possible cross reactivity), 40 kDa (possible cross reactivity)
