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Immunology Innate Immunity Chemokines Alpha Chemokines (CXC)

Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday March 24, 2021

Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19892] to CXCL1/GRO alpha
  • Suitable for: WB
  • Reacts with: Human, Recombinant fragment

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Overview

  • Product name

    Anti-CXCL1/GRO alpha antibody [EPR19892]
    See all CXCL1/GRO alpha primary antibodies
  • Description

    Rabbit monoclonal [EPR19892] to CXCL1/GRO alpha
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human CXCL1/GRO alpha active protein; human GRO beta fragment recombinant protein; human GRO gamma fragment recombinant protein; human fetal lung lysate.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19892
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Alpha Chemokines (CXC)

Images

  • Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)
    Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)
    All lanes : Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411) at 1/1000 dilution

    Lane 1 : Human CXCL1/GRO alpha active protein
    Lane 2 : Human GRO beta fragment recombinant protein
    Lane 3 : Human GRO gamma fragment recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 11 kDa
    Observed band size: 10,36 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Human GRO beta fragment recombinant protein contain aa35-107 with a GST-Tag. Human GRO gamma fragment recombinant protein contain aa35-107 with a His-Tag®. These two protein were made in house.

  • Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)
    Western blot - Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)
    Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411) at 1/100 dilution + Human fetal lung lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 11 kDa
    Observed band size: 11 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)
    Anti-CXCL1/GRO alpha antibody [EPR19892] (ab206411)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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