Anti-Ctk1 antibody (ab47812)
Key features and details
- Rabbit polyclonal to Ctk1
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Overview
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Product name
Anti-Ctk1 antibody -
Description
Rabbit polyclonal to Ctk1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
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Positive control
- This antibody gave a positive signal in S.cerevisiae (Y190) Whole Cell Lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 60 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 60 kDa).Target
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Relevance
Ctk1 is the catalytic subunit of CTDK-1 that hyperphosphorylates the C-terminal repeat domain (CTD) of RNA polymerase II largest subunit. CTDK-I is localized to the nucleolus. Ctk1 also co-precipitates with RNA polymerase I co-indicating a direct interaction between RNA polymerase I and Ctk1. Therefore, CTDK-I appears to play a role regulating distinct nuclear transcriptional machineries. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 853718 Saccharomyces cerevisiae
- SwissProt: Q03957 Saccharomyces cerevisiae
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Alternative names
- CTD kinase 58 kDa subunit antibody
- CTD kinase subunit 1 antibody
- CTD kinase subunit alpha antibody
- CTDK I alpha subunit antibody
Images
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Western blot - Anti-Ctk1 antibody (ab47812)Anti-Ctk1 antibody (ab47812) at 1 µg/ml + S.cerevisiae (Y190) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 120 kDa, 25 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Datasheets and documents
References (0)
ab47812 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-Ctk1 antibody (ab47812)Anti-Ctk1 antibody (ab47812) at 1 µg/ml + S.cerevisiae (Y190) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa, 120 kDa, 25 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes