Anti-Haspin antibody (ab21686)
Key features and details
- Rabbit polyclonal to Haspin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Haspin antibody
See all Haspin primary antibodies -
Description
Rabbit polyclonal to Haspin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Haspin.
Read Abcam's proprietary immunogen policy (Peptide available as ab23532.) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: HeLa Expressing myc-Haspin (interphase); HeLa Expressing myc-Haspin (mitosis) Whole Cell Lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB (1) Use a concentration of 0.2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 88 kDa).Notes WB
Use a concentration of 0.2 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 88 kDa).Target
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Function
Required for normal alignment of chromosomes at metaphase. Phosphorylates histone H3 'Thr-3' during mitosis. -
Tissue specificity
Strongly expressed in testis. Also present in thymus and bone marrow and low levels observed in prostate, intestine, lung, spleen and lymph node. Expressed in fetal skin, liver, kidney and small intestine and also in proliferating but not non-proliferating cell lines. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Haspin subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated on both serine and threonine residues (By similarity). Phosphorylated during mitosis. Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 83903 Human
- Omim: 609240 Human
- SwissProt: Q8TF76 Human
- Unigene: 534059 Human
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Alternative names
- germ cell associated 2 antibody
- Germ cell-specific gene 2 protein antibody
- GSG2 antibody
see all
Images
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Western blot - Anti-Haspin antibody (ab21686)All lanes : Anti-Haspin antibody (ab21686) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2 : HeLa Expressing myc-Haspin (interphase) Whole Cell Lysate - Positive Control at 5 µl
Lane 3 : HeLa Interphase Whole Cell Lysate - Negative Control at 10 µg
Lane 4 : HeLa Expressing myc-Haspin (mitosis) Whole Cell Lysate - Positive Control at 5 µl
Lane 5 : HeLa Mitosis Whole Cell Lysate - Negative Control at 10 µg
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 88 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 23 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Datasheets and documents
References (4)
ab21686 has been referenced in 4 publications.
- Yu F et al. Knockdown of GSG2 inhibits prostate cancer progression in vitro and in vivo. Int J Oncol 57:139-150 (2020). PubMed: 32319597
- Varier RA et al. A phospho/methyl switch at histone H3 regulates TFIID association with mitotic chromosomes. EMBO J 29:3967-78 (2010). PubMed: 20953165
- Markaki Y et al. Phosphorylation of histone H3 at Thr3 is part of a combinatorial pattern that marks and configures mitotic chromatin. J Cell Sci 122:2809-19 (2009). WB ; Human, Mouse . PubMed: 19622635
- Dai J et al. Studies of haspin-depleted cells reveal that spindle-pole integrity in mitosis requires chromosome cohesion. J Cell Sci 122:4168-76 (2009). WB ; Human . PubMed: 19910498
Images
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Western blot - Anti-Haspin antibody (ab21686)All lanes : Anti-Haspin antibody (ab21686) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2 : HeLa Expressing myc-Haspin (interphase) Whole Cell Lysate - Positive Control at 5 µl
Lane 3 : HeLa Interphase Whole Cell Lysate - Negative Control at 10 µg
Lane 4 : HeLa Expressing myc-Haspin (mitosis) Whole Cell Lysate - Positive Control at 5 µl
Lane 5 : HeLa Mitosis Whole Cell Lysate - Negative Control at 10 µg
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 88 kDa
Observed band size: 90 kDa why is the actual band size different from the predicted?
Additional bands at: 23 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
