Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CDCA2 antibody (ab45129)

Key features and details

  • Rabbit polyclonal to CDCA2
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-CDCA2 antibody
    See all CDCA2 primary antibodies

  • Description

    Rabbit polyclonal to CDCA2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 950 to the C-terminus of Human CDCA2.

    Read Abcam's proprietary immunogen policy (Peptide available as ab45267.)

  • Positive control

    • This antibody gave a positive control in the following human lysates: Hela nuclear HepG2 nuclear Jurkat nuclear (data not shown)

  • General notes

     This product was previously labelled as CDCA2, CDCA2

     

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab45129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
Application Abreviews Notes
WB (1)
Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 113 kDa).
ICC/IF
Use a concentration of 5 µg/ml.
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 113 kDa).
ICC/IF
Use a concentration of 5 µg/ml.

Target

  • Relevance

    CDCA2 is a regulator of chromosome structure during mitosis. It is required for condensin-depleted chromosomes to retain their compact architecture through anaphase. It acts by mediating the recruitment of phosphatase PP1-gamma subunit (PPP1CC) to chromatin at anaphase and into the following interphase. At anaphase onset, its association with chromatin targets a pool of PPP1CC to dephosphorylate substrates. CDCA2 is essential for cell viability.

  • Cellular localization

    Nuclear. Excluded from the nucleolus. Present in nucleoplasm throughout the G1, S and G2 stages of the cell cycle. During M phase, it becomes diffuse throughout the cell as the nuclear membrane breaks down, and faintly accumulates later on metaphase chromatin. As the cell progresses to anaphase, it accumulates on chromatin.

  • Database links

    • Alternative names

      • CDCA 2 antibody
      • Cell division cycle associated protein 2 antibody
      • PPP1R81 antibody
      • protein phosphatase 1, regulatory subunit 81 antibody
      • Recruits PP1 onto mitotic chromatin at anaphase protein antibody
      • Repo Man antibody
      see all

    Images

    • Western blot - Anti-CDCA2 antibody (ab45129)
      Western blot - Anti-CDCA2 antibody (ab45129)
      All lanes : Anti-CDCA2 antibody (ab45129) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
      Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 4 : Hep G2 nuclear extract lysate (ab14660)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 113 kDa
      Observed band size: 125 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.

    • Immunocytochemistry/ Immunofluorescence - Anti-CDCA2 antibody (ab45129)
      Immunocytochemistry/ Immunofluorescence - Anti-CDCA2 antibody (ab45129)
      ICC/IF image of ab45129 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45129, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References (2)

    Publishing research using ab45129? Please let us know so that we can cite the reference in this datasheet.

    ab45129 has been referenced in 2 publications.

    • Qian J  et al. Cdk1 orders mitotic events through coordination of a chromosome-associated phosphatase switch. Nat Commun 6:10215 (2015). PubMed: 26674376
    • Wurzenberger C  et al. Sds22 and Repo-Man stabilize chromosome segregation by counteracting Aurora B on anaphase kinetochores. J Cell Biol 198:173-83 (2012). PubMed: 22801782

    Images

    • Western blot - Anti-CDCA2 antibody (ab45129)
      Western blot - Anti-CDCA2 antibody (ab45129)
      All lanes : Anti-CDCA2 antibody (ab45129) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
      Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 4 : Hep G2 nuclear extract lysate (ab14660)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 113 kDa
      Observed band size: 125 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
    • Immunocytochemistry/ Immunofluorescence - Anti-CDCA2 antibody (ab45129)
      Immunocytochemistry/ Immunofluorescence - Anti-CDCA2 antibody (ab45129)
      ICC/IF image of ab45129 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45129, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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