Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CstF-50 antibody [EPR12332] (ab173288)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR12332] to CstF-50
Suitable for: ICC/IF, IHC-P, WB
Reacts with: Human

Product name

Anti-CstF-50 antibody [EPR12332]
See all CstF-50 primary antibodies
Description

Rabbit monoclonal [EPR12332] to CstF-50
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment within Human CstF-50. The exact sequence is proprietary.
Database link: Q05048
Positive control
- 293T, HeLa, A549 and K562 cell lysates; Human cervical carcinoma and testis tissues; A549 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR12332
Isotype

IgG
Research areas

Western blot - Anti-CstF-50 antibody [EPR12332] (ab173288)

All lanes : Anti-CstF-50 antibody [EPR12332] (ab173288) at 1/1000 dilution

Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : A549 cell lysate
Lane 4 : K562 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 48 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CstF-50 antibody [EPR12332] (ab173288)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling CstF-50 with ab173288 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CstF-50 antibody [EPR12332] (ab173288)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling CstF-50 with ab173288 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-CstF-50 antibody [EPR12332] (ab173288)

Immunofluorescent analysis of A549 cells labeling CstF-50 with ab173288 at 1/50 dilution.
Anti-CstF-50 antibody [EPR12332] (ab173288)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com