Anti-CSNK2A1 antibody (ab10466)
Key features and details
- Rabbit polyclonal to CSNK2A1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CSNK2A1 antibody
See all CSNK2A1 primary antibodies -
Description
Rabbit polyclonal to CSNK2A1 -
Host species
Rabbit -
Specificity
Reacts with alpha subunit. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
The immunogen was a synthetic peptide, which represented a portion of human casein kinase 2, alpha 1 polypeptide encoded within exon 3 (LocusLink ID 1457).
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General notes
This product was previously labelled as CKII alpha
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CSNK2A1 antibody (ab10466)
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Western blot - Anti-CSNK2A1 antibody (ab10466) -
Western blot - Anti-CSNK2A1 antibody (ab10466)All lanes : Anti-CSNK2A1 antibody (ab10466) at 1/2500 dilution
Lane 1 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 45 kDa
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Western blot - Anti-CSNK2A1 antibody (ab10466) This image is courtesy of an Abreview submitted by Svetlana KhoronenkovaAll lanes : Anti-CSNK2A1 antibody (ab10466) at 1/5000 dilution (LI-COR Blocker (1:1) in PBS + 0.1% Tween for 3 hours)
Lane 1 : Whole cell lysate of HeLa cells
Lane 2 : siRNA to CK2 alpha in HeLa cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : An Alexa Fluor®680-conjugated Goat anti-rabit polyclonal at 1/10000 dilution
Predicted band size: 45 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Blocking Step: 50% LI-COR® Odyssey® Blocking Buffer for 45 minutes at room temperature.
All non-specific bands were very weak.
