Anti-HN1 antibody (ab247137)
Key features and details
- Rabbit polyclonal to HN1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HN1 antibody
See all HN1 primary antibodies -
Description
Rabbit polyclonal to HN1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human HN1 aa 1-100.
Database link: Q9UK76 -
Positive control
- IHC-P: Human skin, testis and cerebral cortex tissues. ICC/IF: A549 cells. WB: PC-3 cell lysate. A549 cells transfected with control siRNA.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-HN1 antibody (ab247137)
PFA fixed, Triton X-100 permeabilized A549 (Human lung carcinoma cell line) cells labeling HN1 using ab247137 at 4 µg/ml (green) in ICC/IF.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HN1 antibody (ab247137)Paraffin-embedded human skin tissue stained for HN1 using ab247137 at 1/20 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HN1 antibody (ab247137)Paraffin-embedded human testis tissue stained for HN1 using ab247137 at 1/20 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HN1 antibody (ab247137)Paraffin-embedded human cerebral cortex tissue stained for HN1 using ab247137 at 1/20 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HN1 antibody (ab247137)Paraffin-embedded human liver tissue stained for HN1 using ab247137 at 1/20 dilution in immunohistochemical analysis. No positivity in hepatocytes as expected.
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Western blot - Anti-HN1 antibody (ab247137)Anti-HN1 antibody (ab247137) at 0.4 µg/ml + PC-3 (Human prostate adenocarcinoma cell line) cell lysate
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Western blot - Anti-HN1 antibody (ab247137)All lanes : Anti-HN1 antibody (ab247137) at 0.4 µg/ml
Lane 1 : A549 cells transfected with control siRNA
Lane 2 : A549 cells transfected with siRNA probe#1