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Immunology Adaptive Immunity T Cells Non-CD

Anti-CSN8 antibody [EPR5139] (ab124779)

Price and availability

251 280 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-CSN8 antibody [EPR5139] (ab124779)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5139] to CSN8
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-CSN8 antibody [EPR5139]
    See all CSN8 primary antibodies
  • Description

    Rabbit monoclonal [EPR5139] to CSN8
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human CSN8 aa 150-250 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Fetal liver, HepG2, SH SY5Y or HeLa cell lysates IHC-P: Human brain tissue
  • General notes

     This product was previously labelled as COPS8

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5139
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Non-CD
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • SCF Complex E3 Ligase
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Stem Cells
    • Germline Stem Cells
    • Embryonic Germ Cells
    • Developmental Biology
    • Reproduction
    • Germ cell markers

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN8 antibody [EPR5139] (ab124779)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSN8 antibody [EPR5139] (ab124779)

    ab124779, at 1/100, staining CSN8 in formalin fixed paraffin embedded Human brain tissue using immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-CSN8 antibody [EPR5139] (ab124779)
    Western blot - Anti-CSN8 antibody [EPR5139] (ab124779)
    All lanes : Anti-CSN8 antibody [EPR5139] (ab124779) at 1/1000 dilution

    Lane 1 : Fetal liver cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : SH SY5Y cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

    Predicted band size: 23 kDa
    Observed band size: 21 kDa
    why is the actual band size different from the predicted?

  • Anti-CSN8 antibody [EPR5139] (ab124779)
    Anti-CSN8 antibody [EPR5139] (ab124779)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-CSN8 antibody [EPR5139] (ab124779)

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    Alexa Fluor® 488 Anti-CSN8 antibody [EPR5139] (ab208389)

    Applications: ICC/IF

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    HRP Anti-CSN8 antibody [EPR5139] (ab208842)

    Applications: WB

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    Anti-CSN8 antibody (ab228695)

    Applications: ICC/IF, IHC-P

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