Anti-CRMP1 antibody (ab36199)
Key features and details
- Rabbit polyclonal to CRMP1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CRMP1 antibody
See all CRMP1 primary antibodies -
Description
Rabbit polyclonal to CRMP1 -
Host species
Rabbit -
Specificity
No cross reactivity was detected with CRMP2, CRMP3, CRMP4 or CRMP5. -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
A 15 residue synthetic peptide derived from C-terminal domain of human collapsing-response mediator.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CRMP1 antibody (ab36199)All lanes : Anti-CRMP1 antibody (ab36199)
Lane 1 : E.coli lysate transformed with cDNA of human CRMP1.
Lane 2 : E.coli lysate transformed with cDNA of human CRMP2.
Lane 3 : E.coli lysate transformed with cDNA of human CRMP3.
Lane 4 : E.coli lysate transformed with cDNA of human CRMP4.
Lane 5 : E.coli lysate transformed with cDNA of human CRMP5.
Predicted band size: 62 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody (ab36199)Ab36199 staining human CRMP1 in olfactory receptor neurons within the olfactory mucosa by immunohistochemistry. -
Immunocytochemistry/ Immunofluorescence - Anti-CRMP1 antibody (ab36199)ICC/IF image of ab36199 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36199, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
