Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Friday March 05, 2021

Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14520(B)] to CRMP1 - N-terminal
Suitable for: WB, IHC-P
Reacts with: Human

Product name

Anti-CRMP1 antibody [EPR14520(B)] - N-terminal
See all CRMP1 primary antibodies
Description

Rabbit monoclonal [EPR14520(B)] to CRMP1 - N-terminal
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human CRMP1 aa 50-150 (N terminal). The exact sequence is proprietary.
Database link: Q14194
Positive control
- Human cerebellum lysate, Fetal brain lysate, SH-SY5Y cell lysate, Paraffin-embedded Human brain sections.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR14520(B)
Isotype

IgG
Research areas

Western blot - Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)

All lanes : Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517) at 1/20000 dilution

Lane 1 : SH-SY5Y lysate
Lane 2 : Fetal brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

Predicted band size: 62 kDa
Western blot - Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)

Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517) at 1/5000 dilution + Human cerebellum lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

Predicted band size: 62 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling CRMP1 with ab180517 at 1/500 dilution. Anti-rabbit HRP seconday antibody was used and counterstaining with Hematoxylin was performed.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Anti-CRMP1 antibody [EPR14520(B)] - N-terminal (ab180517)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com