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Anti-Crk p38 antibody [EPR4984(2)] - BSA and Azide free (ab248562)

Anti-Crk p38 antibody [EPR4984(2)] - BSA and Azide free (ab248562)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4984(2)] to Crk p38 - BSA and Azide free
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Crk p38 antibody [EPR4984(2)] - BSA and Azide free
    See all Crk p38 primary antibodies
  • Description

    Rabbit monoclonal [EPR4984(2)] to Crk p38 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    The immunogen used for this product shares 79%  identity (78% positives) with CRKL. Cross-reactivity with this protein has not been confirmed experimentally.

  • Tested applications

    Suitable for: Flow Cyt, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248562 is the carrier-free version of ab133581. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248562 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR4984(2)
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Cancer
    • Signal transduction
    • Other

Images

  • Flow Cytometry - Anti-Crk p38 antibody [EPR4984(2)] - BSA and Azide free (ab248562)
    Flow Cytometry - Anti-Crk p38 antibody [EPR4984(2)] - BSA and Azide free (ab248562)
    This data was developed using ab133581, the same antibody clone in a different buffer formulation.ab133581, at a 1/100, staining Crk p38 in permeabilized HepG2 cells using flow cytometry analysis (Red). Green curve corresponds to a control Rabbit IgG antibody (negative control).
  • Western blot - Anti-Crk p38 antibody [EPR4984(2)] - BSA and Azide free (ab248562)
    Western blot - Anti-Crk p38 antibody [EPR4984(2)] - BSA and Azide free (ab248562)
    All lanes : Anti-Crk p38 antibody [EPR4984(2)] (ab133581) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 33 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab133581, the same antibody clone in a different buffer formulation.

     

  • Anti-Crk p38 antibody [EPR4984(2)] - BSA and Azide free (ab248562)
    Anti-Crk p38 antibody [EPR4984(2)] - BSA and Azide free (ab248562)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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