Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23841-83] to GIRK2 - BSA and Azide free
  • Suitable for: WB, IP, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free
    See all GIRK2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23841-83] to GIRK2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, IHC-P, IHC-Frmore details
    Unsuitable for: Flow Cyt (Intra)

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse, rat and human brain lysate. IHC-P: Human cerebrum and insulinoma tissue. Mouse and rat cerebellum tissue. IHC-Fr: Mouse and rat cerebral cortex, cerebellum and hippocampus tissue. IP: Mouse brain tissue lysate.

  • General notes

    ab281831 is the carrier-free version of ab259909. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab281831 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR23841-83

  • Isotype

    IgG

Images

  • Western blot - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)
    Western blot - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)
    All lanes : Anti-GIRK2 antibody [EPR23841-83] (ab259909) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate at 20 µg
    Lane 2 : Mouse liver tissue lysate at 40 µg
    Lane 3 : Rat brain tissue lysate at 20 µg
    Lane 4 : Rat liver tissue lysate at 40 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 48 kDa



    This data was developed using ab259909, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Samples are non-boiled as boiling may cause protein aggregates.

    Negative control: liver (PMID: 8929423).

    Exposure time: 5.5 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)

    This data was developed using ab259909, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling GIRK2 with ab259909 at 1/200 (2.345 µg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab259909 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Cytoplasmic staining on human cerebrum (PMID: 8642402). 

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)
    Immunohistochemistry (Frozen sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)

    This data was developed using ab259909, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebral cortex tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).

    Cytoplasmic staining on mouse cerebral cortex (PMID: 8929423) is observed.

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunoprecipitation - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)
    Immunoprecipitation - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)

    This data was developed using ab259909, the same antibody clone in a different buffer formulation.

    GIRK2 was immunoprecipitated from 0.35 mg mouse brain tissue lysate 10 µg with ab259909 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab259909 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse brain tissue lysate 10 µg.

    Lane 2: ab259909 IP in mouse brain tissue lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259909 in mouse brain tissue lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 23 seconds.

    Input sample is non-boiled as boiling may cause protein aggregates.The expression of truncated GIRK2 proteins observed around 40KD is consistent with what has been described in the literature (PMID:28951616).

  • Western blot - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)
    Western blot - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)
    All lanes : Anti-GIRK2 antibody [EPR23841-83] (ab259909) at 1/1000 dilution

    Lane 1 : Human brain tissue lysate at 20 µg
    Lane 2 : Human liver tissue lysate at 40 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 48 kDa
    Observed band size: 48,40,35 kDa why is the actual band size different from the predicted?



    This data was developed using ab259909, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Molecular weight around 40kDa, cleaved forms. (PMID:28951616).

    Samples are non-boiled as boiling may cause protein aggregates.

    Negative control: liver (PMID: 8929423).

    Exposure time: 5.5 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)

    This data was developed using ab259909, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human insulinoma tissue labelling GIRK2 with ab259909 at 1/200 (2.345 µg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab259909 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Cytoplasmic staining on human insulinoma (PMID: 7592809). 

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)

    This data was developed using ab259909, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labelling GIRK2 with ab259909 at 1/200 (2.345 µg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab259909 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Postive staining predominantly on granular layer of mouse cerebellum (PMID: 8642402, PMID: 9023358 ).

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)

    This data was developed using ab259909, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labelling GIRK2 with ab259909 at 1/200 (2.345 µg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab259909 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Postive staining predominantly on granular layer of rat cerebellum (PMID: 8642402, PMID: 9023358).

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)

    This data was developed using ab259909, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human liver tissue labelling GIRK2 with ab259909 at 1/200 (2.345 µg/ml) dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab259909 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Negative control: No staining on human liver (PMID: 7592809). 

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)
    Immunohistochemistry (Frozen sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)

    This data was developed using ab259909, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).

    Cytoplasmic staining on mouse cerebellum (PMID: 8929423) is observed.

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)
    Immunohistochemistry (Frozen sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)

    This data was developed using ab259909, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).

    Cytoplasmic staining on mouse hippocampus (PMID: 8929423) is observed.

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)
    Immunohistochemistry (Frozen sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)

    This data was developed using ab259909, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).

    Negative control: No staining on mouse liver (PMID: 7592809) is observed. 

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)
    Immunohistochemistry (Frozen sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)

    This data was developed using ab259909, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebral cortex tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).

    Cytoplasmic staining on rat cerebral cortex (PMID: 8929423) is observed.

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)
    Immunohistochemistry (Frozen sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)

    This data was developed using ab259909, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebellum tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).

    Cytoplasmic staining on rat cerebellum (PMID: 8929423) is observed.

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)
    Immunohistochemistry (Frozen sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)

    This data was developed using ab259909, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat hippocampus tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).

    Cytoplasmic staining on rat hippocampus (PMID: 8929423) is observed.

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)
    Immunohistochemistry (Frozen sections) - Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)

    This data was developed using ab259909, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver tissue labeling GIRK2 with ab259909 at 1/100 (4.69 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). The nuclear counterstain was DAPI (Blue).

    Negative control: No staining on rat liver (PMID: 7592809) is observed. 

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)
    Anti-GIRK2 antibody [EPR23841-83] - BSA and Azide free (ab281831)

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