Anti-Cripto1/CRIPTO antibody [EPNCIR106A] - BSA and Azide free (ab247653)
![Anti-Cripto1/CRIPTO antibody [EPNCIR106A] - BSA and Azide free (ab247653)](https://www.abcam.com/ps/products/247/ab247653/Images/ab247653-5-anti-cripto1-cripto-antibody-epncir106a-bsa-and-azide-free-flow-cytometry.jpg "Anti-Cripto1/CRIPTO antibody [EPNCIR106A] - BSA and Azide free (ab247653)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR106A] to Cripto1/CRIPTO - BSA and Azide free
- Suitable for: ICC, Flow Cyt, WB, IP
- Reacts with: Human
Overview
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Product name
Anti-Cripto1/CRIPTO antibody [EPNCIR106A] - BSA and Azide free
See all Cripto1/CRIPTO primary antibodies -
Description
Rabbit monoclonal [EPNCIR106A] to Cripto1/CRIPTO - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, Flow Cyt, WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab247653 is the carrier-free version of ab108391 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab247653 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of David Solomon. View antibodies from NCI Center for Cancer Research Collaboration.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPNCIR106A -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] - BSA and Azide free (ab247653)This data was developed using ab108391, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of NCCIT (human pluripotent embryonal carcinoma) cells labeling Cripto1/CRIPTO with purified ab108391
at 1/2200 dilution (1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. -
![Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] - BSA and Azide free (ab247653)](https://www.abcam.com/ps/products/247/ab247653/Images/ab247653-408020-anti-cripto1-cripto-antibody-epncir106a-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] - BSA and Azide free (ab247653)Anti-Cripto1/CRIPTO antibody [EPNCIR106A] (ab108391) at 1/2000 dilution (purified) + NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 21 kDaThis data was developed using ab108391, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] - BSA and Azide free (ab247653)](https://www.abcam.com/ps/products/247/ab247653/Images/ab247653-3-anti-cripto1-cripto-antibody-epncir106a-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] - BSA and Azide free (ab247653)This data was developed using ab108391, the same antibody clone in a different buffer formulation.ab108391 (purified) at 1:30 dilution (2ug) immunoprecipitating Cripto1/CRIPTO in NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate.
Lane 1 (input): NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+): ab108391 & NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab108391 in NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution. -
![Immunocytochemistry - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] - BSA and Azide free (ab247653)](https://www.abcam.com/ps/products/247/ab247653/Images/ab247653-2-anti-cripto1-cripto-antibody-epncir106a-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] - BSA and Azide free (ab247653)This data was developed using ab108391, the same antibody clone in a different buffer formulation.Immunocytochemistry/ Immunofluorescence analysis of NCCIT (Human pluripotent embryonic carcinoma epithelial cell) cells labeling Cripto1/CRIPTO with Purified ab108391 at 1:500 dilution (1.1μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] - BSA and Azide free (ab247653)](https://www.abcam.com/ps/products/247/ab247653/Images/ab247653-398112-anti-cripto1-cripto-antibody-epncir106a-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Cripto1/CRIPTO antibody [EPNCIR106A] - BSA and Azide free (ab247653) -
![Anti-Cripto1/CRIPTO antibody [EPNCIR106A] - BSA and Azide free (ab247653)](https://www.abcam.com/ps/products/247/ab247653/Images/ab247653-6-benefits-of-recombinant-antibodies.png)
Anti-Cripto1/CRIPTO antibody [EPNCIR106A] - BSA and Azide free (ab247653)
