Anti-CPSF2/CPSF100 antibody (ab81553)
Key features and details
- Rabbit polyclonal to CPSF2/CPSF100
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CPSF2/CPSF100 antibody
See all CPSF2/CPSF100 primary antibodies -
Description
Rabbit polyclonal to CPSF2/CPSF100 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Xenopus tropicalis
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Immunogen
Synthetic peptide corresponding to Human CPSF2/CPSF100.
Database link: NP_059133.1 -
Positive control
- HeLa, 293T and NIH3T3 whole cell lysates.
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General notes
This product was previously labelled as CPSF2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPSF2/CPSF100 antibody (ab81553)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic islet cell tumor (left) and mouse teratoma (right) tissues labelling CPSF2/CPSF100 with ab81553 at 1/200 (1µg/ml). Detection: DAB.
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Western blot - Anti-CPSF2/CPSF100 antibody (ab81553)All lanes : Anti-CPSF2/CPSF100 antibody (ab81553) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 88 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
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Immunoprecipitation - Anti-CPSF2/CPSF100 antibody (ab81553)HeLa cell lysate (1mg) was immunoprecipitated using ab81553 at 3µg/mg (lane 1) or IgG control (lane 2). 20% of the immunoprecipitate was analysed by Western blotting using ab81553 at 1µg/ml. Bands were observed by chemiluminescence with a 3 second exposure time.
