Anti-CPE antibody [EPR23306-21] (ab275882)
![Anti-CPE antibody [EPR23306-21] (ab275882)](https://www.abcam.com/ps/products/275/ab275882/Images/ab275882-1-anti-cpe-antibody-epr2330621-immunohistochemistry-human-pancreas.jpg "Anti-CPE antibody [EPR23306-21] (ab275882)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23306-21] to CPE
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Human
Overview
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Product name
Anti-CPE antibody [EPR23306-21]
See all CPE primary antibodies -
Description
Rabbit monoclonal [EPR23306-21] to CPE -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-Fr MouseIHC-P MouseHuman
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human and mouse pancreas tissue. IHC-Fr: Mouse pancreas tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23306-21 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPE antibody [EPR23306-21] (ab275882)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling CPE with ab275882 at 1/2000 (0.266 µg/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human pancreas islets is observed (PMID:21152387). The section was incubated with ab275882 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Frozen sections) - Anti-CPE antibody [EPR23306-21] (ab275882)](https://www.abcam.com/ps/products/275/ab275882/Images/ab275882-3-anti-cpe-antibody-epr2330621-immunohistochemistry-frozen-mouse-pancreas.jpg)
Immunohistochemistry (Frozen sections) - Anti-CPE antibody [EPR23306-21] (ab275882)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse pancreas tissue labeling CPE with ab275882 at 1/100 (5.31 µg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Green). Positive staining on mouse pancreatic islet. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPE antibody [EPR23306-21] (ab275882)](https://www.abcam.com/ps/products/275/ab275882/Images/ab275882-2-anti-cpe-antibody-epr2330621-immunohistochemistry-mouse-pancreas.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CPE antibody [EPR23306-21] (ab275882)Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling CPE with ab275882 at 1/2000 (0.266 µg/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on mouse islets is observed (PMID:21152387). The section was incubated with ab275882 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Anti-CPE antibody [EPR23306-21] (ab275882)](https://www.abcam.com/ps/products/275/ab275882/Images/ab275882-4-benefits-of-recombinant-antibodies.png)
Anti-CPE antibody [EPR23306-21] (ab275882)