Anti-COX7A2L antibody (ab66107)
Key features and details
- Rabbit polyclonal to COX7A2L
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-COX7A2L antibody
See all COX7A2L primary antibodies -
Description
Rabbit polyclonal to COX7A2L -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human COX7A2L.
Read Abcam's proprietary immunogen policy (Peptide available as ab69103.) -
Positive control
- This antibody gave a positive signal in the following Human Whole Cell lysates: TE 671 Jurkat - Staurosporine Treated (24hr, 500nM) HeLa HepG2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab66107 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatCowApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 13 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 13 kDa).Target
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Function
May be a regulatory subunit of cytochrome c oxidase that mediates the higher level of energy production in target cells by estrogen. -
Sequence similarities
Belongs to the cytochrome c oxidase VIIa family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 9167 Human
- Entrez Gene: 20463 Mouse
- Entrez Gene: 298762 Rat
- Omim: 605771 Human
- SwissProt: O14548 Human
- SwissProt: Q61387 Mouse
- Unigene: 339639 Human
- Unigene: 741313 Human
see all -
Alternative names
- COX7a related protein antibody
- COX7a-related protein antibody
- COX7A2L antibody
see all
Images
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Western blot - Anti-COX7A2L antibody (ab66107)All lanes : Anti-COX7A2L antibody (ab66107) at 1 µg/ml
Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 13 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
References (1)
ab66107 has been referenced in 1 publication.
- Jian C et al. Deficiency of PHB complex impairs respiratory supercomplex formation and activates mitochondrial flashes. J Cell Sci 130:2620-2630 (2017). PubMed: 28630166
Images
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Western blot - Anti-COX7A2L antibody (ab66107)All lanes : Anti-COX7A2L antibody (ab66107) at 1 µg/ml
Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 13 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
