Anti-COX1 / Cyclooxygenase 1 antibody (ab84602)
Key features and details
- Rabbit polyclonal to COX1 / Cyclooxygenase 1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-COX1 / Cyclooxygenase 1 antibody
See all COX1 / Cyclooxygenase 1 primary antibodies -
Description
Rabbit polyclonal to COX1 / Cyclooxygenase 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human COX1/ Cyclooxygenase 1 aa 550 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab93495) -
Positive control
- This antibody gave a positive signal in the following human whole cell lysates: A431; HUVEC.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanApplication Abreviews Notes WB Notes Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 68 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.Target
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Function
May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells. -
Pathway
Lipid metabolism; prostaglandin biosynthesis. -
Sequence similarities
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 5742 Human
- Omim: 176805 Human
- SwissProt: P23219 Human
- Unigene: 201978 Human
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Alternative names
- COX 1 antibody
- COX 3 antibody
- COX-1 antibody
see all
Images
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Western blot - Anti-COX1 / Cyclooxygenase 1 antibody (ab84602)All lanes : Anti-COX1 / Cyclooxygenase 1 antibody (ab84602) at 1 µg/ml
Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 71,73 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
This antibody was raised against an immunogen that is predicted to recognize both the short and long isofroms of Human Prostaglandin G/H synthase 1 precursor (COX1). The predicted molecular weights of the short and long isoforms are 68 kDa and 64 kDa respectively. However, COX1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
References (0)
ab84602 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-COX1 / Cyclooxygenase 1 antibody (ab84602)All lanes : Anti-COX1 / Cyclooxygenase 1 antibody (ab84602) at 1 µg/ml
Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 68 kDa
Observed band size: 71,73 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
This antibody was raised against an immunogen that is predicted to recognize both the short and long isofroms of Human Prostaglandin G/H synthase 1 precursor (COX1). The predicted molecular weights of the short and long isoforms are 68 kDa and 64 kDa respectively. However, COX1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
