Anti-COTL1 antibody (ab235833)
Key features and details
- Rabbit polyclonal to COTL1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-COTL1 antibody
See all COTL1 primary antibodies -
Description
Rabbit polyclonal to COTL1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
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Immunogen
Recombinant full length protein corresponding to Human COTL1 aa 1 to the C-terminus.
Database link: Q14019 -
Positive control
- WB: Mouse brain, kidney and thymus lysate; HeLa, HepG2, U-87 MG, HL-60 and A549 whole cell lysate. IHC-P: Human lung cancer and lymphoid tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300 -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-COTL1 antibody (ab235833)All lanes : Anti-COTL1 antibody (ab235833) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5 : Mouse thymus tissue lysate
Lane 6 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 7 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate
Lane 8 : A549 (human lung carcinoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COTL1 antibody (ab235833)Paraffin-embedded human lymphoid tissue stained for COTL1 using ab235833 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COTL1 antibody (ab235833)Paraffin-embedded human lung cancer tissue stained for COTL1 using ab235833 at 1/100 dilution in immunohistochemical analysis.
